血浆蛋白质组——人类蛋白质组计划的“探路者”  

Plasma Proteome:A “Pathfinder” of Human Proteome Project

作　　者：王英[1] 赵晓航[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《生物化学与生物物理进展》2004年第8期673-678,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 ( 3 0 3 70 713 ;3 0 2 2 5 0 45和 3 0 1710 49);国家高技术"863"计划资助项目 ( 2 0 0 4AA2 2 70 60 )~~

摘　　要：概述了血浆蛋白的研究现状、难点和策略 .血浆是血液中无形的液体成分 ,是一种十分复杂和多样化的基质 ,包含数百万种蛋白质和小分子多肽、盐、类脂、氨基酸和糖等 .血浆蛋白参与机体免疫、凝血 抗凝血、物质运输、营养和对生长信号调节等多种重要的生理功能 .人体器官的病理变化可导致血浆蛋白在结构和数量上的改变 ,这种特征性的变化对疾病诊断和疗效监测具有十分重要的意义 .然而 ,迄今为止人类对血浆蛋白的了解还十分有限 ,只有很少一部分血浆蛋白被用于常规的临床诊断 .全面而系统地认识健康和疾病状态下血液循环中血浆蛋白的性质 ,会极大地加速对具有疾病诊断和治疗监测作用的血浆标志蛋白的研发 .国际人类蛋白质组组织于 2 0 0 2年首先选择了血浆蛋白质组作为人类蛋白质组首期执行计划之一 ,其初期目标是 :a 比较各种蛋白质组分析技术平台的优点和局限性 ;b 用这些技术平台分析人类血浆和血清的参考样本 ;cBlood plasma is a complex body fluid that contains various proteins and small molecules including peptides, salts, lipids, amino acids and sugars. Plasma proteins perform different kinds of housekeeping functions, such as immunoresponse, coagulation, anticoagulation, transportation, nutrition and regulating signaling cascades, which normally are altered both in structure and amount during pathogenesis. These characteristics of plasma proteome are critical for disease diagnosis and therapeutic monitoring. Nevertheless, only a handful of proteins are currently well known and used in routine clinical diagnosis. A comprehensive, systematic characterization of circulating proteins and peptides in health and disease will greatly facilitate development of biomarkers for many human diseases. The human proteome organization has launched the Human Plasma Proteome Project as one of the major initiatives since April 2002. In the pilot phase of the project, as a 'pathfinder' of human proteomics study, the major goals are ( 1) to compare the advantages and limitations of many technology platforms; ( 2) to compare reference specimens of human plasma (EDTA, heparin, citrate-anti-coagulated) and serum prepared specifically for this project with the technology platforms; and (3) to create a knowledge base. The brief introduction about the plasma proteome project including research status, major problems faced and technical strategies been used are reviewed.

关 键 词：血浆 蛋白质组 人类 血浆蛋白 生物标志 质谱 

分 类 号：Q51[生物学—生物化学]