端粒单链结合蛋白hPOT1核定位结构域的鉴定  

Identification of nuclear localization sequence of hPOT1

作　　者：林坚[1] 陈皓[1] 白云秀[1] 金蕊[1] 张彬[1] 黄翠芬[1] 黄君健[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第4期319-321,336,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究发展计划 ("973"计划 )资助项目 (G2 0 0 0 0 5 70 0 1);国家自然科学基金资助项目( 3 0 3 70 70 41)

摘　　要：目的 :鉴定端粒单链结合蛋白hPOT1核定位结构域。方法 :通过hPOT1的不同长度缺失突变体与EGFP绿色荧光蛋白融合 ,转染细胞后用荧光显微镜观察融合蛋白在细胞内的定位。结果 :确定了hPOT1进核序列是定位于第 35 5～ 375位氨基酸之间的富含碱性氨基酸序列 ,其中R3 72 ,R3 73 影响hPOT1的细胞核内定位。结论 :鉴定出hPOT1的核定位结构域 ,该结构域影响了hPOT1不同形式剪接体蛋白的细胞内分布。为进一步研究hPOT1在端粒末端调控中的作用提供了线索。Objective: To identify the nuclear localization sequence of human POT1 (protection of telomeres). Methods: Full length and truncated or mutated forms of hPOT1 were fused to EGFP and examined for their influence on subcellular localization by transient expression in HeLa cells. Results: Residues 355-375 were necessary for nuclear localization of hPOT1. Amino acid R 372 and R 373 facilitated its nuclear localization, indicating that these basic amino acids played a significant role in this process. Conclusion: The nuclear localization sequence of hPOT1 is between 355-375 residues. Some splicing forms of hPOT1 lack this nuclear localization sequence, which may influence their subcellular location and the ability of binding the telomere.

关 键 词：HPOT1 进核序列 端粒 DNA结合蛋白质类 

分 类 号：Q51[生物学—生物化学]