咪唑啉-1受体哺乳动物细胞稳定表达系统的建立  

Stable expression of imidazoline-1 receptor in mammalian cells

作　　者：赵琰[1] 苏瑞斌[1] 吴宁[1] 徐波[1] 刘茵[1] 路新强[1] 李锦[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第4期333-336,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究发展计划 ("973"计划 )资助项目 ( 2 0 0 3CB5 15 40 0 )

摘　　要：目的 :在中国仓鼠卵巢 (CHO)细胞上稳定转染咪唑啉 1受体。方法 :用脂质体介导方法将I1R蛋白cDNA稳定转染到缺乏天然I1R的CHO细胞中。结果 :建立了单独稳定转染I1R而无α2 肾上腺素受体表达的细胞系 ,为独立考察I1R的功能和信号转导通路提供了理想的细胞模型。结论 :成功建立了稳定表达咪唑啉 1受体的细胞模型。Objective: To stably transfect imidazoline-1 receptor (I 1R) in Chinese hamster ovary (CHO) cells. Methods: I 1R Protein cDNA was stably transfected in CHO cells by the method of lipofectamine. Results: The cell line that stably expressed I 1R without the expression of α 2-adrenergic receptors was constructed. This cell line provided a perfect cell model to investigate the function and signal transduction pathway of I 1R. Conclusion: A cell line with stable expression of I 1R was constructed.

关 键 词：咪唑啉类 仓鼠属 卵巢 CHO细胞 转染 

分 类 号：R-332[医药卫生]