肝动脉阻断结合外放射治疗大鼠肝移植瘤的初步观察  

Preliminary Study on The Effect of Hepatic Arterial Occlusion Combined with External Radiation in The Treatment for Rat Liver Transplantated Cancer

作　　者：郭伟剑[1] 白永瑞[1] 冯艺[1] 庄军燕[1] 陆长虹[1] 

机构地区：[1]上海第二医科大学附属新华医院,上海200092

出　　处：《肿瘤学杂志》2004年第5期315-317,共3页Journal of Chinese Oncology

基　　金：上海市科技发展基金资助(004119086)

摘　　要：[目的]观察肝动脉阻断法结合外放射治疗肝肿瘤的效果。[方法]采用大鼠肝内移植Walker256肿瘤模型,以肝动脉结扎(HAL)的方法阻断肝动脉血供。荷瘤大鼠分为对照组、HAL组、HAL+外放射(RT)组。观察治疗后肝内肿瘤的大小变化,原位杂交法检测肿瘤组织VEGFmRNA表达水平,免疫组化法检测肿瘤组织PCNA表达。[结果]对照组、HAL组、HAL+RT组肿瘤体积分别为(0.88±0.33)cm3、(0.53±0.43)cm3、(0.33±0.32)cm3,HAL组和HAL+RT组的肿瘤生长抑制率分别为39.8%、62.5%。HAL+RT组的肿瘤体积较对照组明显缩小(P<0.01),而且,HAL+RT组PCNA及VEGFmRNA表达水平与对照组相比明显下降(P<0.05)。[结论]肝动脉阻断结合外放射综合治疗较单独的动脉阻断治疗可提高治疗效果。To observe the effect of hepatic arterial occlusion combined with external radiation for rat liver transplanted cancer. Walker 256 carcinosarcoma was transplanted into rat's liver to establish liver cancer model. Hepatic arterial ligation(HAL) was used to block the hepatic arterial blood supply, and simulate hepatic arterial embolization. Rats with bearing tumor were divided into 3 groups: control group, HAL group, HAL combined with radiotherapy (HAL+RT) group. Tumor volume and tumor growth inhibiting rate after therapy were evaluated. The expression of VEGF mRNA was detected by in situ hybridization. The expression of PCNA was detected by immunohistochemistry. The mean tumor volume in control group, HAL group, and HAL+RT group was 0.88+0.33cm3, 0.53+0.43cm3, 0.33+0.32cm3, respectively. The tumor growth inhibiting rage in the later two groups was 39.8%, and 62.5%, respectively. The tumor volume in the HAL+RT group was smaller than that of the control group (P<0.01). The expression of VEGF mRNA and PCNA decreased significantly in HAL+RT group, compared with that of control group(P<0.05). [Conclusion] Hepatic arterial occlusion combined with radiotherapy is more effective than hepatic arterial occlusion alone in treatment for rat transplanted liver cancer.

关 键 词：肝肿瘤 肝动脉栓塞 放射疗法 血管内皮生长因子 

分 类 号：R73-3[医药卫生—肿瘤] R735.7[医药卫生—临床医学]