β1-整合素反义寡核苷酸抑制胃癌细胞系SGC7901粘附侵袭的实验研究  

Inhibition of Cell Attachment and Invasion of Human Gastric Cancer Cell line SGC7901 by Integrinβ1 asODN

作　　者：董武[1] 崔越宏[2] 孙宏[1] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤科,沈阳市110001 [2]复旦大学附属中山医院肿瘤治疗中心化疗科

出　　处：《中国肿瘤临床》2004年第11期601-603,共3页Chinese Journal of Clinical Oncology

基　　金：本文课题受国家"973"重大基础研究项目;辽宁省教育厅重点项目基金资助(编号:G1998051203;2001225002)

摘　　要：目的:观察β1-整合素反义寡核苷酸对高侵袭性人胃癌细胞SGC7901粘附侵袭的抑制作用。方法:以脂质体为载体将硫代磷酸修饰的反义寡核苷酸转染SGC7901细胞,免疫化学方法观察转染后以Biotin标记的Inte鄄grinβ1asODN在细胞内的分布;RT-PCR及流式细胞术分别测定Integrinβ1mRNA和蛋白表达变化;MTT法和Boy鄄den小室模型分别测定其粘附力和侵袭力。结果:Integrinβ1asODN转染SGC7901后1h,胞浆及胞核内可见均匀分布的浅棕色颗粒,随着时间的延长,颗粒颜色不断加深;RT-PCR结果显示489bp处条带不同程度变浅;Integrinβ1蛋白表达曲线明显左移;粘附和侵袭实验证明,Integrinβ1asODN转染的SGC7901细胞的粘附力及侵袭力明显下降(P<0.001),抑制率分别为54%和76%,明显优于随机序列(P<0.001)。结论:Integrinβ1asODN转染SGC7901可降低其mRNA和蛋白表达水平,从而抑制其对细胞外基质(ECM)的粘附和侵袭。Objective: To observe the inhibition of adhesion and invasion of SGC7901 to ECM by Integrinβ1 asODN. Methods: ODN were transfected into SGC7901, taking liposome as vector. The distribution of ODN was recorded by immunochemistry, changes in the expression of Integrinβ1 mRNA and protein were determined by RT-PCR and FCM, respectively. The adhesion and invasion to ECM were measured by MTT and Boyden chamber method,respectively. Results: After Integrinβ1 asODN was transfected into SGC7901 it distributed evenly in cytoplasm and nucleus. PCR and FCM revealed a weakened band on 489bp and a shift-left curve,respectively. Adhesion and invasion assay also presented a decrease,with the inhibiting rate of 54% and 76%. The extent of decrease induced by Integrinβ1 asODN was larger than that by the random ODN(P<0.001). Conclusion: Transfection of Integrinβ1 asODN into SGC7901 induced a decrease in the expression of Integrinβ1 mRNA and protein, thus a decrease in adhesion and invasion to ECM, with a better effect than random ODN.

关 键 词：胃癌 INTEGRINΒ1 反义寡核苷酸(as ODN) 

分 类 号：R735.2[医药卫生—肿瘤]