格拉诺赛特与化疗药物联合动员自体外周血干细胞的临床研究  

Mobilization of Autologous Peripheral Blood Stem Cells by Chemotherapy and Granulocyte (Recombinant Human Granulocyte Colony-stimulating Factor)

作　　者：艾斌[1] 石远凯[1] 杨建良[1] 何小慧[1] 周生余[1] 韩晓红[1] 刘鹏[1] 周爱萍[1] 张长弓[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院内科,北京市100021

出　　处：《中国肿瘤临床》2004年第11期635-637,共3页Chinese Journal of Clinical Oncology

摘　　要：目的:观察格拉诺赛特联合化疗对恶性实体瘤患者自体外周血造血干细胞(APBSC)的动员效果。方法:32例患者进入本研究,其中恶性淋巴瘤18例,乳腺癌11例,生殖细胞肿瘤3例。自化疗后白细胞降至最低点时开始皮下注射格拉诺赛特150～250μg/day1,至APBSC采集结束前一天,白细胞恢复到5.0×109/L以上时开始连日采集APBSC;当累计采集的单核细胞≥5.0×108/kg或CD34+细胞≥2.0×106/kg时停止采集。结果:开始给予格拉诺赛特及开始采集APBSC的中位时间分别为化疗开始后的第12(5～15)天和第15(13～20)天,格拉诺赛特的中位给药次数为5(3～12)次,全组患者平均采集到的单核细胞及CD34+细胞总数分别为5.76±2.05×108/kg和15.58±10.36×106/kg,平均粒-单集落形成单位21.01±20.75×104/kg。动员过程中不良反应轻微,此后30例接受移植者全部获得造血功能重建。结论:格拉诺赛特联合化疗是一种安全、有效的APBSC的动员方法,国人采用150～250μg/day1的剂量即可得到满意的动员效果。Objective: To observe the effect of Granulocyte (recombinant human granulocyte colony-stimulating factor) and chemotherapy on autologous peripheral blood stem cells (APBSC) mobilization of patients with malignant solid tumors. Methods: This study includes 32 patients with solid tumor (malignant lymphoma 18, breast cancer 11 and germ cell tumors 3). The Granulocyte (Chugai Pharmaceutical Co, Ltd ) was given as a daily injecting subcutanously at the dose of 150～250?滋g body-1 day-1 from the day of the nadir of white blood cell(WBC) to the day before the end of APBSC harvest. APBSC apheresis was performed when WBC ≥5.0×109/L from the nadir and finished when accumulated MNC≥5×108/kg or CD34+ cells count≥2×106/kg. Results: The median beginning time of Granulocyte given and apheresis was the day 12 (5～15) and the day 16 (13～20) from the begin of chemotherapy respectively. The median times of Granulocyte given was 5 (4～12). The total count of MNC and CD34+ cells harvested for each patient were 5.76±2.05×108/kg and 15.58±10.36×106/kg, respectively. Colony forming ability of PBSC were done and the mean CFU-GM was 21.01±20.75×104/kg. A few side effects were observed in the mobilization. All 30 patients who underwent APBSCT achieved hematopoiesis recovery. Conclusion: Granulocyte combining with chemotherapy was a safe and highly effective method for APBSC mobilization. The dose of 150～250?滋g body-1 day-1 of Granulocyt combining with chemotherapy were suitable for APBSC mobilization in Chinese patients.

关 键 词：RHG-CSF 实体瘤 自体外周血干细胞动员 

分 类 号：R730.5[医药卫生—肿瘤]