9-顺维甲酸对人肺癌组织RAR_α、RAR_β及RXR_α转录的影响  

Effects of 9-cis retinoic acid on RAR_α、RAR_ and RXR_α transcription in human lung tumor tissue

作　　者：刘晓黎[1] 戴洪海[2] 贺美兰[1] 田鹏[3] 胡国强[4] 于雪艳[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012 [2]山东省肿瘤医院放射治疗科,济南250000 [3]济南空军总医院胸外科,山东济南250000 [4]山东大学齐鲁医院胸外科,山东济南250012

出　　处：《山东大学学报（医学版）》2004年第3期257-260,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(39770730)

摘　　要：目的:观察经9-顺维甲酸(9-cis-RA)处理前后肺癌组织和对照肺组织维甲酸受体α?β及维甲酸类受体α转录水平的变化,以进一步探讨维甲酸抑瘤的分子机制?方法:用组织块法培养肺癌组织和对照肺组织,用RT-PCR技术检测RARα?RARβ及RXRα的mRNA水平?结果:肺癌组织的RARα和RXRα基础转录水平高于对照肺组织,而RARβ基础转录水平低于对照肺组织;应用9-cis-RA 24 h后对照肺组织RARα转录水平升高;肺癌组织RARβ转录水平升高(P<0.01),与对照肺组织基础转录水平相近?结论:肺癌组织中RARβ受体表达异常可能与肺癌发生有关;9-cis-RA可诱导肺癌组织RARβ的表达显著升高,达对照肺组织mRNA相同表达水平?Objective: To observe the effects of 9-cis-retinoic acid(9-cis-RA) on RARα?RARβ and RXRα transcriptional levels in lung tumor tissue and control lung tissue. Methods: Tumor tissue and lung tissue were cultured by tissue mass method. RARα?RARβ and RXRα transcriptional levels were detected by RT-PCR. Results: Compared with control tissue, tumor tissue has a significant increase of RARα transcription(P<0.01), significant decrease of RARβ transcription (P<0.01) and increase of RXRα transcription (P<0.05); When treated with 9-cis-RA, RARα transcription was increased in control tissue (P<0.05) while RARβ significantly increased in tumor tissue (P<0.01). Conclusion: There is a significant lower expression of RARβ in lung tumor tissue than in control,which comes from the same person; 9-cis-RA can induce RARβ expression significantly, reaching the transcriptional level in control lung tissue. [

关 键 词：维甲酸 肺肿瘤 受体 维甲酸 

分 类 号：R734.2[医药卫生—肿瘤]