骨肉瘤中MMP-2和PCNA表达的意义及相关关系  

Significance and relationships of expressions of MMP-2 and PCNA in osteosarcoma

作　　者：云文科[1] 赵子会 王瑞铎 任希存[1] 邵斌[3] 

机构地区：[1]武警内蒙古总队医院外二科,呼和浩特010030 [2]武警内蒙古总队后勤部卫生处,呼和浩特010010 [3]内蒙古医学院第一附属医院骨科,呼和浩特010020

出　　处：《武警医学》2004年第6期424-427,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的　探讨基质金属蛋白酶 - 2 (MMP - 2 )、增殖细胞核抗原 (PCNA)在骨肉瘤中表达的意义和临床价值。方法采用免疫组织化学S -P法测取 32例骨肉瘤、2 0例骨软骨瘤标本的MMP - 2、PCNA表达值 ,以SPSS10 .0软件行数据处理 ,分析其表达的意义和相关性。结果　MMP - 2阳性表达率在骨肉瘤为 81.2 5 % ,在骨软骨瘤为 0 ,两者差异显著 (P <0 .0 1) ;在骨肉瘤中 ,MMP - 2阳性表达组的软组织浸润或转移比率为 73.0 8% ,明显高于阴性表达组 (P <0 .0 5 ) ;PCNA的阳性表达率在骨肉瘤中为 84 .38% ,在骨软骨瘤中为 10 .0 0 % ,两者差异显著 (P <0 .0 1) ;在骨肉瘤中 ,MMP - 2与PCNA呈正相关 (rs =+0 .70 3,P <0 .0 5 )。结论　MMP - 2的高表达可能与骨肉瘤浸润转移密切相关 。Objective To study the significance and clinical value of expressions of matrix metlloproteinase-2(MMP-2) and proliferating cell nuclear antigen(PCNA) in osteosarcoma.Methods MMP-2 and PCNA were detected for the specimens of 32 osteosarcomas and 20 osteochondromas by immunohistochemical S-P method and SPSS 10.0 software was used to analyze the significance and relationships of their expressions.Results The positive rate of MMP-2 was 81.25 percent in osteosarcoma group and zero in osteochondroma group with a significant difference(P<0.01). In osteosarcoma, the rate of invasion or metastasis was 73.08% in MMP-2 positive group,significantly higher than (16.67%) in negatively expressed group (P<0.05). The positive expression rate of PCNA was 84.38% in osteosarcoma group and 10.00% in osteochondroma group,also with a significant difference(P<0.01). In osteosarcoma group, the expression of MMP-2 was positively correlated with the expression of PCNA (r_s=+0.703,P<0.05).Conclusion High expression of MMP-2 is closely correlated with invasion and metastasis of osteosarcoma. PCNA is one of markers of osteosarcoma proliferation.

关 键 词：骨肉瘤 MMP-2 PCNA 基质金属蛋白酶-2 增殖细胞核抗原 

分 类 号：R738.1[医药卫生—肿瘤]