实验性哮喘小鼠T淋巴细胞对B细胞分泌特异性IgE的调节作用  

Regulatory effect of T lymphocyte on specific-IgE secreted by B lymphocyte in mouse with asthma

作　　者：胡素贤[1] 黄瑾[2] 

机构地区：[1]天津港口医院呼吸科,天津300456 [2]吉林大学第二医院呼吸科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2004年第4期515-517,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (30 0 70 32 9)

摘　　要：目的 :探讨支气管哮喘发病中 T细胞对 B细胞产生特异性 Ig E (SIg E)的影响。方法 :建立小鼠支气管哮喘模型 ,分离小鼠脾脏 T、 B细胞 ,分组进行细胞培养 ,观察哮喘鼠 T细胞对 B细胞分泌 SIg E的影响 ,并以EL ISA法检测不同组合细胞培养上清中 SIg E。结果 :在有或没有特异性抗原参与时 ,哮喘鼠 B细胞分泌 SIg E水平均明显高于对照组 B细胞 (P<0 .0 5 ) ;自身 T细胞均可降低哮喘鼠 B细胞分泌 SIg E的水平 (P<0 .0 5 ) ;无论特异性抗原是否参与 ,从数值上看 ,哮喘鼠 T细胞有使对照鼠 B细胞 SIg E分泌水平增加趋势 ,但差异无显著性(P>0 .0 5 )。结论 :T细胞在支气管哮喘的发病过程中 ,尤其是 SIg E的产生中起重要的调节作用 ;T细胞功能异常是哮喘发病的原因之一。Objective To study the regulation and interference of T lymphocyte on antigen-specific IgE (SIgE) antibodies by B lymphocyte in bronchial asthma mouse model and clarify the role of T lymphocyte in the pathogenesis of asthma. Methods The asthma mouse model was set up at first, then T and B lymhphocytes were isolated from the mice spleen and were cultivated in different combinations. The supernates were collected and the specific anti-OVA antibody IgE in the supernate were tested with enzyme linked immunosorbent assay (ELISA). Results The level of SIgE secreted by B cells from asthma mouse was higher than that from the control mouse (P<0.05). After regulated by own T lymphocytes, the level of SIgE secreted by B cells from asthma mouse decreased significantly (P<0.05). With or without specific antigen, after regulated by T cells from asthma mouse, the level of SIgE secreted by B cells from the control mouse increased, but there was no significant difference (P>0.05). Conclusion T lymphocyte has a key role in regulating and controlling the pathogenesis of asthma. And T cell functional abnormality is one of the important mechanisms of asthma.

关 键 词：哮喘 免疫球蛋白E T淋巴细胞 B淋巴细胞 疾病模型 动物 

分 类 号：R-332[医药卫生] Q25[生物学—细胞生物学]