维生素A干预对大鼠抗氧化能力及细胞膜流动性影响的研究  

Effect of different dosage of vitamin A supplement on anti-oxidative activity and the fluidity of erythrocyte membrane in rats

作　　者：韩磊[1] 马爱国[2] 张燕[3] 

机构地区：[1]青岛大学医学院附属医院,青岛266003 [2]青岛大学医学院医学营养研究所 [3]青岛海慈医疗集团内分泌糖尿病医院

出　　处：《卫生研究》2004年第4期450-452,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 0 70 659)

摘　　要：目的　通过对大鼠补充不同剂量维生素A(VA)观察机体抗氧化能力及对红细胞膜流动性的影响 ,了解抗氧化活性的最佳剂量。方法　将Wistar大鼠随机分为 4组 ,分别为维生素A缺乏组 (VA1组 )补充1 1 4 3(VA2组 )、4 2 86 (VA3组 )和 1 4 2 86 μgRE·kg- 1 ·d- 1 (VA4组 )维生素A三个剂量组。测定各组大鼠血浆VA含量、SOD、MDA和GSH Px的含量和活性 ,用荧光偏振度P值和微粘度 η值评价红细胞膜的流动性。 结果　各组血浆中VA水平随补充剂量的增加而增加。血浆中SOD、GSH -Px和MDA的结果显示 ,补充剂量为VA2、VA3组SOD的水平较VA缺乏组和VA4组明显偏低 (P <0 0 1 )。VA3组的MDA较其它 3个剂量组明显下降 (P <0 0 1 )。VA4组的SOD、MDA含量明显高于其它三个剂量组 (P <0 0 1 )。VA3组血浆中GSH -Px的活性明显升高 ,P <0 0 1。细胞膜流动性的结果显示 :VA3组P值最小 (P <0 0 1 ) ;VA4组P值明显增高 (P<0 0 1 )。微粘度 η在VA3组与VA缺乏组和VA2组之间未见明显差异 ,与VA4组相比明显下降 (P <0 0 1 )。结论　维生素A补充剂量为 4 2 86 μgRE·kg- 1 ·d- 1 时 ,能够发挥较好抗氧化作用 ,红细胞膜的流动性明显增加 ,过量摄入维生素A可造成机体的中毒反应 。Objective To investigating the effect of vitamin A (VA)at different dosage of vitamin A on anti oxidative activity and the fluidity of erythrocyte membrane in rats and safe dosage Methods Wistar rats selected for VA supplement trial were randomly divided into four groups The first group was no VA supplement as a control, and the second, third, forth groups were daily supplemented with VA 11 43,42 86 and 142 86μg RE/kg respectively. The trial lasted 8 weeks.Vitamin A was dissolved in soybean oil and daily given to animals by gavage. The blood samples were collected at the end of the trial. The level of plasma VA was analyzed by fluorescent spectrometry. SOD,GSH and MDA in plasma were analyzed. The blood erythrocyte membrane fluidity was detected by fluorescence polarization method. Results The results showed that plasma retinol levels increaseed with increas of vitamin A. After 8 week supplement, SOD activities in the second and the third groups were lower than those of the first and the fourth group( P <0 01). which was the highest in the four groups( P <0 01).The level of plasma MDA in the third was the lowest( P <0 01). GSH Px activity was higher in the third group than other three groups( P <0 01). Moreover, P (fluorescence polarization) was lower in 42 86μg RE/kg group than other three groups, and P significantly increased in the fourth group( P <0 01),which indicates that the erythrocyte membrane fluidity increased significantly after 42 86μg RE/kg vitamin A supplement.Conclusion The supplement of 42 86μg RE/kg VA could improve anti oxidative activity and enhance erythrocyte membrane fluidity. Overtake vitamin A could produce a poisoning effect and a stronger oxidative damage.

关 键 词：视黄醇 抗氧化 膜流动性 超氧化物歧化酶 

分 类 号：Q562[生物学—生物化学] Q735