Bcl-xL反义寡核苷酸对裸鼠人鼻咽癌移植瘤化疗药物敏感性的影响  

Synergetic effect of antisense oligodeoxynucleotides and chemotheraphy in transplanted human nasopharyngeal carcinoma of nude mice

作　　者：闵玲[1] 周克元[2] 梁统[2] 

机构地区：[1]广东省妇幼保健院检验科,广东广州510010 [2]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《实用肿瘤杂志》2004年第4期285-287,共3页Journal of Practical Oncology

基　　金：广东省教育厅"千百 +工程优秀人才培养基金"(2 0 0 0 4 3)

摘　　要：目的　观察 Bcl- x L 反义寡核苷酸 (ASODN)对裸鼠人鼻咽癌移植瘤化疗药物顺铂敏感性的影响。方法　建立裸鼠人鼻咽癌移植瘤模型 ,分为对照组、ASODN治疗组、顺铂 (DDP)治疗组、ASODN+DDP联合治疗组 ,观察裸鼠瘤体变化及组织形态学改变。结果　单独应用 ASODN和 DDP治疗组的抑瘤率分别为 14 .2 %和 30 .9% ,而在相同条件下 ,联合用药组效果最明显 ,抑瘤率为 5 2 .2 % ,较 ASODN、DDP单独应用差异有显著性。结论　ASODN与 DDP联合化疗有协同作用 ,ASODN可以提高肿瘤细胞对顺铂的敏感性 。Objective To investigate effects of antisense oligodeoxynucleotides (ASODN) on the chemotherapeutic drug sensitivity of transplantd human nasopharyngeal carcinoma(NPC) in nude mice.Methods Human NPC was transplanted in nude mice,and the tumor bearing mice were divided into four groups: control group, ASODN group, DDP group and ASODN plus DDP group. The weight of subcutaneous tumors was measured, and the morphology of tumor cells observed microscopically.Results The inhibition rate of single-agent ASODN and DDP was 14.2% and 30.9%,respectively. In contrast, the combined treatment with ASODN plus DDP led to a dramatic reduction in tumor volume with a inhibition rate of 52 2%.Conclusion ASODN can markedly enhance the sensitivity of NPC xenograft cells to DDP,presenting a synergistic antitumor activity with DDP.

关 键 词：鼻咽肿瘤/病理学 寡核苷酸类 反义 顺铂/治疗应用 原癌基因蛋白质类 基因疗法 脱噬作用 疾病模型 动物 

分 类 号：R739.63[医药卫生—肿瘤]