FK506胶原药膜抑制兔角膜移植的免疫排斥反应  

FK506 delivery collagen membrane suppression on immunologic rejection in rabbit of corneal transplantation

作　　者：周文天[1] 俞益丰[1] 邓青[1] 黄旭[1] 

机构地区：[1]江西医学院第二附属医院眼科,江西南昌330006

出　　处：《眼科新进展》2004年第4期254-256,共3页Recent Advances in Ophthalmology

基　　金：江西省卫生厅基金资助 (编号 :0 30 1 0 52 )~~

摘　　要：目的　探讨FK5 0 6胶原药膜治疗和预防兔高危角膜移植术后免疫排斥反应的有效性和可行性。方法　 2 4只新西兰大白兔建立严重的碱烧伤的高危角膜移植动物模型 ,然后施行穿透性角膜移植术 ,将全部大白兔的右眼定为实验组、左眼定为对照组 ,术后右眼用缓释胶原药膜载FK5 0 6给药 ,左眼局部滴FK5 0 6眼液 ,用酶联免疫吸附 (ELISA)方法测定术后 1、2、4、8、2 4、72h的角膜、结膜、房水的药物浓度和持续时间 ;观察植片不同时期的角膜移植排斥反应发生率 ,平均随访 3个月。结果　实验组 1h角膜浓度 (84 .1±2 0 .6 )ng·g-1、结膜浓度 (2 9.2± 10 .3)ng·g-1、房水浓度 (2 5 .6± 6 .8)ng·L-1,4h后分别为 (2 30 .2± 2 6 .6 )、(15 4 .5± 19.4 )、(15 .3± 5 .7)ng·L-1;72h后分别为 (2 3.5± 11.2 )、(13.8± 9.3)、(11.2± 2 .4 )ng·L-1,而对照组的 1h角膜、结膜、房水的药物浓度分别为(4 1.2± 11.6 )、(30 .5± 10 .5 )、(8.2± 2 .1)ng·L-1,4h后分别为 (17 8± 6 .5 )、(18.5±6 3)、(6 .7± 1.2 )ng·L-1;8h以后检测到药物浓度非常低 ,不能达到有效药物浓度。在实验期间 ,实验组的植片排斥反应的发生率及上皮愈合质量 2个指标均明显优于对照组。结论　FK5 0Objective To evaluate the efficacy and feasibility of FK506 drug delivery collagen membrane for prevention and treatment of immunologic rejection corneal grafts in rabbit.Methods High-risk corneal graft rabbits model was by passing severity alkali burnning in 24 New Zealand rabbits and received corneal grafts.Twenty four animal models (48 eyes) were divided into 2 groups: right eyes with FK506 collagen membrane groups(experiment group) and left eyes with FK506 groups(control group).At 1 hour,2,4,8,24,72 hours,FK506 concentration and maintained period were measured by ELISA in the cornea,conjunctiva,aqueous humor. The average 3 follow-up months.Incidence of immunologic rejection were observed.Results The mean FK506 concentration of experiment group in cornea,conjunctiva,aqueous humor in 1 hour were (84.1±20.6),(29.2±10.3) and (25.6±6.8)ng·L -1 ;in 4 hours were (230.2±26.6),(154.5±19.4 ) and (25.3±5.7) ng·L -1 ;in 72 hours were (23.5±11.2)、(13.8 ±9.3) and (11.2±2.4 )ng·L -1 .The mean FK506 concentration of control groups in cornea,conjunctiva,aqueous humor in 1 hour were (41.2±11.6)、(30.5±10.5 ) and (8.2±2.1)ng·L -1 ;in 4 hour were (17.8±6.5)、(18.5±6.3) and (6.7±1.2)ng·L -1 ;in 8 hours was not FK506 concentration;In follow-up period the experiment group corneal was significantly better than control groups in graft rejection incidence,epithelial concrescence quality.Conclusion The FK506 drug delivery collagen membrane can significantly improve FK506 concentration in cornea,conjunctiva,aqueous humor and maintain a high concentration for a long period ,which has strong effects on prevention and treatment of immunologic rejection of corneal grafts. It is a safe and effective method for clinic application.

关 键 词：FK506缓释胶原药膜 角膜移植 排斥反应 酶联免疫吸附法 

分 类 号：R779.65[医药卫生—眼科]