TGF-β_1AS-ODN对牛眼小梁细胞TGF-β_1、纤维连接蛋白、Ⅰ型胶原表达的影响  

Effects of TGF-β_1 AS-ODN on the expression of TGF-β_1 ,fibronectin and collagen-Ⅰin bovine trabecular meshwork cells

作　　者：陈飞[1] 魏厚仁[1] 曾艳彩[2] 吕源淑[1] 张樱[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,湖北武汉430022 [2]广州军区武汉总医院信息科,湖北武汉430070

出　　处：《眼科新进展》2004年第4期260-263,共4页Recent Advances in Ophthalmology

摘　　要：目的　观察转化生长因子 β1(transforminggrowthfactor β1,TGF β1)反义寡核苷酸对牛眼小梁细胞 (bovinetrabecularmeshworkcells,BTMC)表达TGF β1、纤维连接蛋白、Ⅰ型胶原的影响。方法　脂质体介导下 ,将 0 .1,0 .5 ,1μmol·L-1TGF β1反义寡核苷酸作用于BTMC ,对照组分别加入 1μmol·L-1TGF β1正义寡核苷酸脂质体复合物、2 0g·L-1脂质体、无血清RPMI 16 4 0。然后用半定量RT PCR检测TGF β1反义寡核苷酸对BTMC表达TGF β1、纤维连接蛋白、Ⅰ型胶原的影响。结果　与无血清RPMI 16 4 0对照组相比 ,0 .1μmol·L-1TGF β1反义寡核苷酸即可抑制BTMC表达TGF β1,0 .5 ,1μmol·L-1TGF β1反义寡核苷酸可完全抑制BTMC表达TGF β1(P <0 .0 5 )。 0 .1,0 .5 μmol·L-1TGF β1反义寡核苷酸可浓度依赖性抑制BTMC表达纤维连接蛋白、Ⅰ型胶原 ,1μmol·L-1TGF β1反义寡核苷酸可完全抑制BTMC表达纤维连接蛋白、Ⅰ型胶原 (P <0 .0 5 )。 2 0 g·L-1脂质体、1μmol·L-1TGF β1正义寡核苷酸不能抑制BTMC表达TGF β1、纤维连接蛋白、Ⅰ型胶原 (P >0 .0 5 )。结论　进一步证实TGF β1可通过自分泌方式作用BTMC ,促进细胞外基质的合成。通过TGF β1反义寡核苷酸抑制BTMC表达TGF β1、纤维连接蛋白、Ⅰ型胶原 ,Objective To observe the effects of antisense oligonucleotides against transforming growth factor-β_1(TGF-β_1)on the expression of TGF-β_1,fibronectin and collagen-Ⅰin bovine trabecular meshwork cells(BTMC).Methods After lipofectemine 2000 reagent delivery,BTMC were exposed to 0.1,0.5,1μmol·L -1 antisense TGF-β_1,1μmol·L -1 sense TGF-β_1,20g·L -1 lipofectemine,serum-free RPMI-1640 respectively.The semi-quantitative RT-PCR was used for detecting the effect of antisense TGF-β_1 on the expression of TGF-β_1,fibronectin,and collagen-Ⅰ.Results In comparison with RPMI-1640 control group,0.1μmol·L -1 antisense TGF-β_1 could inhibit the TGF-β_1 expression in BTMC which was completely inhibited at 0.5,1μmol·L -1 concentrations(P<0.05).With 0.1,0.5μmol·L -1 antisense TGF-β_1 could dose-dependently inhibit the fibronectin,collagen-Ⅰexpression in BTMC which was completely inhibited at 1μmol·L -1 concentration(P<0.05).These effects weren't observed in 20 g·L -1 lipofectemine and 1μmol·L -1 sense TGF-β_1.Conclusion It is further confirmed that TGF-β_1 may promote the extracellular marix synthesis through autocrine effects on BTMC. Antisense TGF-β_1 may be expected to treat primary open-angle glaucoma on its pathogenesis through inhibition of the expression of TGF-β_1,fibronectin and collagen-Ⅰ.

关 键 词：原发性开角型青光眼 小梁细胞 转化生长因子 转染 反义寡核苷酸 

分 类 号：R773.3[医药卫生—眼科]