上皮钙黏附蛋白、黏附分子CD44H、基质金属蛋白酶-3、肿瘤转移抑制基因nm23H_1和血管内皮生长因子的表达与鼻咽癌转移的关系  

Expression and correlation with metastasis of E-cadherin,CD44H,matrix metalloproteinase-3,nm23H_1 and VEGF in nasopharyngeal carcinoma

作　　者：王双乐[1] 江远仕[1] 黄河澄[1] 杨强[2] 

机构地区：[1]汕头市中心医院耳鼻咽喉头颈外科,广东汕头515031 [2]武汉大学人民医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉科杂志》2004年第8期470-472,共3页Journal of Clinical Otorhinolaryngology

基　　金：广东省汕头市重点科技计划基金资助项目 (No:99 3)

摘　　要：目的 :探讨上皮钙黏附蛋白 (E cadherin)、黏附分子CD4 4H、基质金属蛋白酶 3(MMP 3)、肿瘤转移抑制基因nm2 3H1和血管内皮生长因子 (VEGF)基因的表达与鼻咽癌颈淋巴结转移及远处转移的关系。方法 :应用免疫组织化学SP法检测 6 2例鼻咽癌组织 (鼻咽癌组 )和 4 0例正常鼻咽黏膜组织 (对照组 )中E cadherin、CD4 4H、MMP 3、nm2 3H1和VEGF的表达。结果 :在鼻咽癌有淋巴结转移组中 ,E cadherin和nm2 3H1的表达减弱 ,CD4 4H和MMP 3的表达增强 ,与无淋巴结转移组的表达差异有统计学意义 ,VEGF在两组的表达差异无统计学意义 ;E cadherin与CD4 4H、MMP 3的表达分别呈负相关关系 ,CD4 4H与MMP 3的表达呈正相关 ;在鼻咽癌远处转移组中 ,E cadherin和nm2 3H1的表达减弱 ,CD4 4H、MMP 3和VEGF的表达均增强 ,与无远处转移组比较 ,差异均有统计学意义 ;CD4 4H与VEGF的表达呈正相关关系。结论 :E cadherin、CD4 4H、MMP 3和nm2 3H1与鼻咽癌颈淋巴结转移及远处转移的关系密切 ,VEGF仅与鼻咽癌颈淋巴结转移相关 ,它们是肿瘤转移的重要因素 ,可作为评估鼻咽癌转移的生物学指标。Objective:To investigate the expression of E-cadherin, CD44H, matrix metalloproteinase-3, nm23H 1 and VEGF in nasopharyngeal carcinoma and its relationship to the metastasis.Method:The expression of E-cadherin, CD44H, matrix metalloproteinase-3, nm23H 1 and VEGF in 62 patients with nasopharyngeal carcinoma was detected by immunohistochemical SP method.Result:In the group of nasopharyngeal carcinoma with lymph node metastasis, the expression of E-cadherin and nm23H 1 reduced and the expression of CD44H and MMP-3 increased,and there were respectively significant difference compared to the group of non-lymph node metastasis, but there wasn′t significant difference between the two groups in VEGF. Furthermore, expression of E-cadherin was inversely correlated with that of CD44H and MMP-3,and the expression of CD44H was positively correlated with that of MMP-3.In the group of nasopharyngeal carcinoma with distant metastasis, the expression of E-cadherin and nm23H 1 also increased and the expression of CD44H, MMP-3 and VEGF all increased.Compared with the group of negative distant metastasis, there were respectively significant difference. In addition, there was a positive correlation between the expression of CD44H and VEGF.Conclusion:E-cadherin, CD44H, matrix metalloproteinase-3 and nm23H 1 genes are closely related with lymph node and distant metastasis of nasopharyngeal carcinoma, while VEGF gene only relates to lymph node metastasis. They are important factors actting on metastasis of neoplasms.

关 键 词：鼻咽肿瘤 E-钙黏附素 基质金属蛋白酶 基因 

分 类 号：R739.63[医药卫生—肿瘤]