人类XRCC_1-399单核苷酸多态性对原发性肝癌的影响  

Influence of human XRCC_1-399 single nucleotide polymorphism on primary hepatocytic carcinoma

作　　者：杨金龙[1] 韩永年[1] 郑水根[1] 

机构地区：[1]上海市第八人民医院肝病研究室,上海200233

出　　处：《肿瘤》2004年第4期322-324,共3页Tumor

摘　　要：目的　探讨人类DNA修复基因XRCC1 399单核苷酸多态性 (SNP)在HBV感染者发生原发性肝细胞癌 (HCC)中的作用。方法　 72例HCC患者经病理检查证实 ,根据地缘、性别、年龄按 1∶1～ 2比例匹配 ,选择 137例对照者。采用聚合酶链反应 限制片段长度多态性 (PCR RFLP)技术检测受试者XRCC1基因第 399位SNP。结果　单一人类XRCC1 399SNP因素与HCC的发生无关。在XRCC1 399Arg/Arg型受试者中 ,HBV感染与否与HCC的发生无关 (P =0 .2 70 ) ,但在Gln/Gln型和Arg/Gln型受试者中 ,伴HBV感染者较不伴HBV感染者更容易发生HCC[2 5 .7%对 5 .3% ,Mantel HaenszelOR估计值为 2 .5 6 3,95 %可信区间 (CI)为 1.190～ 5 .5 18,P =0 .0 16 ]。结论　人类XRCC1 399位氨基酸突变在HBV阳性人群的HCC的发生中可能具有一定作用。Objective To investigate the correlation between a single nucleotide polymorphism (SNP) of XRCC 1 -399, a human NDA repair gene, and the development of primary hepatocarcinoma (HCC) in a case-control study. Methods The XRCC 1 -399 SNP was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 72 HCC patients with pathological evidences and 137 matched control subjects, who were recruited by being matched on living area, sex and age. Results Human XRCC 1 -399 SNP alone was not associated with the risk of HCC development. Among individuals with the wild type of XRCC 1 -399 Arg/Arg, being with or without HBV infection did not affect the risk of HCC development; whereas of individuals with mutative types Gln/Gln or Arg/Gln, those connected with HBV infection had a greater risk than those not connected with HBV infection (25.7% v.s. 5.3%, Mantel-Haenszel OR 2.563, 95% confidence interval 1.190～5.518, P =0.016). Conclusion Human XRCC 1 -399 SNP may be contributed to the HCC development in individuals with HBV infection.

关 键 词：XRCC1-399单核苷酸 多态性 原发性肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]