PDGF-B链基因三链形成寡核苷酸对C_6胶质瘤细胞增殖和细胞周期的影响  

The effects of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C_6 glioma cells

作　　者：李维方[1] 周定标[2] 余新光[2] 金由辛[3] 

机构地区：[1]解放军第306医院神经外科 [2]解放军总医院神经外科 [3]中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子生物学国家重点实验室

出　　处：《肿瘤》2004年第4期336-339,共4页Tumor

基　　金：全军"十五"医药卫生科研基金(编号 :0 1MB0 66)

摘　　要：目的　观察PDGF B链基因三链形成寡核苷酸 (triplex formingoligonucleotide ,TFO)对C6胶质瘤细胞增殖和细胞周期的影响。方法　应用免疫荧光流式细胞技术观察PDGF B链基因TFO对C6胶质瘤细胞PDGF B、PCNA表达的影响。应用流式细胞技术观察PDGF B链基因TFO对C6胶质瘤细胞细胞周期的影响。结果　PDGF B链基因TFO对C6胶质瘤细胞PDGF B链基因、PCNA的表达有明显抑制作用 ,而且抑制作用存在浓度依赖性。PDGF B链基因TFO能使C6胶质瘤细胞S期的百分率明显降低 ,阻止细胞由静止期 (G0 G1期 )进入 (S期 )。结论　PDGF B链基因TFO能够抑制C6胶质瘤细胞PDGF B链基因的表达 ,阻碍细胞进入S期 。Objective To clarify effect of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C 6 glioma cells. Methods The expressions of PDGF-B chain and PCNA in C 6 glioma cells after adding a set of dosages of TFO were quantified by immunofluorescence flow cytometric analysis.Cell cycle of C 6 glioma cells after adding a set of dosages of TFO were detected by flow cytometric analysis. Results TFO can obviously inhibit the expressions of PDGF-B chain and PCNA in C 6 glioma cells in concentration-dependent fashion. TFO can obviously inhibit the transit of cells from the G 0 -G 1 to S phase in concentration-dependent fashion. Conclusion TFO can effectively suppress the PDGF-B chain expression and the cell proliferation C 6 glioma cells. TFO also can effectively induce the C 6 glioma cells arrest in the G 1 phase of the cell cycle.

关 键 词：血小板源生长因子 细胞增殖 细胞周期 三链形成寡聚脱氧核苷酸 C6胶质瘤细胞 

分 类 号：R739.41[医药卫生—肿瘤] R73-362[医药卫生—临床医学]