肿瘤易感基因101在胃癌细胞中的表达及意义  

Expression and its significance of tumor susceptibility gene 101(TSG101) in gastric cancer cell lines

作　　者：申慧琴[1] 韩者艺[1] 韩霜[1] 薛妍[1] 扎西措拇[1] 胡家露[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化疾病研究所,西安710032

出　　处：《肿瘤》2004年第4期345-348,共4页Tumor

基　　金：创新研究群体基金资助项目 (3 0 0 2 40 0 2 )

摘　　要：目的　探讨肿瘤易感基因 10 1(TSG10 1)在不同胃癌细胞系中的表达及其意义。方法　采用巢式RT PCR、DNA重组技术和Westernblot等方法从mRNA和蛋白水平检测TSG10 1的表达。结果　在 7株胃癌细胞系中均有野生型TSG10 1表达 ,经测序分析无基因突变 ;此外 ,尚存在 1个 2 88bp的剪接变异体 ;在胃癌细胞系MKN4 5、SGC790 1及其耐药亚系SGC790 1/VCR和SGC790 1/ADR中 ,还发现 1个 874bp的剪接变异体 ;并发现野生型TSG10 1在SGC790 1/VCR和SGC790 1/ADR中的表达较亲本细胞SGC790 1增高。结论　TSG10 1及其剪接变异体的表达在胃癌细胞系中常见 ,野生型TSG10 1可能参与了胃癌多药耐药的发生。Objective To study the expression and significance of tumor susceptibility gene 101(TSG101) in different gastric cancer cell lines. Methods The expression levels of TSG101 mRNA and protein were determined using nested RT-PCR、DNA recombinant technology and western blot in gastric cancer cell lines. Results The full-length TSG101 and 288bp aberrant spliced variants were detected in all gastric cancer cell lines. Mutations of the full-length TSG101 were not found by DNA sequence analysis and its expression level was higher in SGC7901/VCR and SGC7901/ADR than in SGC7901. Another 874bp aberrant splicing variant was detected in gastric cancer cell lines MKN45, SGC7901 and its multidrug resistance sublines SGC7901/VCR, SGC7901/ADR. Conclusion The expressions of the full-length TSG101 and its spliced variants may be common in gastric cancer cell lines. The full-length TSG101 may participate in multidrug resistance of gastric cancer.

关 键 词：肿瘤易感基因101 胃肿瘤 肿瘤细胞 培养的 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]