两种引物PCR法检测宫颈刮片中HPV DNA的对比及意义  

The comparative study on HPV DNA test for cervical scraping smears with PCR mediated by two different primers

作　　者：邬素芳[1] 徐茜[1] 陈刚[1] 王薇[1] 辜海念[1] 卢运萍[1] 周利平[1] 杜娟[1] 李辅军[1] 廖国宁[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《肿瘤》2004年第4期410-412,共3页Tumor

基　　金：国家杰出青年基金 (3 0 0 2 5 0 17);国家重点基础研究发展规划项目(2 0 0 2CB5 13 10 0 )

摘　　要：目的　探讨聚合酶链式反应检测宫颈刮片中人乳头瘤病毒的意义及可行性。方法　共检测 131例宫颈刮片 ,分为门诊组和宫颈癌组。每例分别使用通用型引物和 16型E7引物进行PCR ,比较两组间及两对引物检测的阳性率。结果　普查组和宫颈癌组阳性率分别为 32 .99%和 73.5 3% ,两组阳性率比较 ,差异有极其显著性 (P <0 .0 0 1)。普查组中 ,通用引物和HPV16E7引物扩增阳性率分别为 2 7.84 %和 16 .4 9% ,差异无显著性 (P >0 .0 5 )。宫颈癌组中 ,通用引物和HPV16E7引物扩增阳性率分别为 38.2 4 %和 6 7.6 5 % ,差异有显著性 (P <0 .0 5 )。结论　PCR法检测宫颈刮片中的人乳头瘤病毒为一种可行的方法。通用型引物在普通人群中检测的敏感性好 ,可以作为一种筛查的方法 。Objective To investigate the reliability and feasibility of human papillomavirus (HPV) DNA test for cervical scraping smears with polymerase chain reaction (PCR).Methods One hundred and thirty one cases of cervical scraping specimens were collected,the positive rates and accuracy of HPV infection were determined in outpatients and cervical cancer patients.GP5+/GP6+ and E7 primer pairs designed for detecting HPV L1 and HPV type 16 E7 were tested in this study.Results Positive rates of HPV DNA in outpatients and cervical cancer patients,32.99% and 73.53% respectively,showed significant statistica difference( P <0.001).In outpatients,detection rates of HPV DNA with GP5+/GP6+ and E7 primer pairs were 27.84% and 16.49% respectively,statistical analysis revealed no significant difference( P >0.05).However,in cervical cancer patients,detection rates were 38.24% and 67.65%,respecfively,showed significant difference( P <0.05).Conclusion HPV DNA test of cervical scraping smears is feasible with PCR.GP5+/GP6+ primer pairs may be a useful probe to screen HPV infection in popular population.It is suggested that high risk type HPV DNA test is very important in cervical cancer high risk populations.

关 键 词：人乳头瘤病毒 聚合酶链反应 子宫颈癌 诊断 

分 类 号：R737.33[医药卫生—肿瘤]