假丝酵母菌对高浓度油脂废水的降解性能  

Rapid biodegradation of wastewater containing highly concentrated lipids with a new Candida strain

作　　者：胡玉洁[1] 郭兴要[1] 杨鹏[1] 刘妮妮[1] 王璋[1] 

机构地区：[1]中国食品发酵工业研究院,北京100027

出　　处：《工业水处理》2004年第8期46-51,共6页Industrial Water Treatment

摘　　要：对本实验室分离筛选的优良油脂分解菌株——假丝酵母WZFF.G-284的各种微生物学和生化分析试验结果表明,该菌株对于高含油废水具有很强的适应能力,在以高浓度食用色拉油作为唯一碳源、添加适当无机盐的实际工业废水开放处理体系中,该菌株生长繁殖力强,能迅速形成优势菌群,分解油脂。该菌株除菌体细胞具有高强乳化作用能力外,还可合成乳化性能强的生物乳化剂达21.5g/L,同时生产68.3U/mL的高稳定性胞外脂肪酶。该微生物净化处理技术可以在高负荷下持续稳定运转,对油脂、COD和BOD的降解速度快,去除率均在95%以上,分解效果显著。An efficient yeast strain, Candida sp. WZFF.G - 284 isolated from an active sludge and preserved in our laboratory, has been investigated for its ability to biodegrade wastewaters containing highly concentrated lipids by the experiments using an actual wastewater from a vegetable oil factory in Beijing in which salad oil is added as the sole carbon source and some simple inorganic salts in an open environment without any sterilization. The results from the microbiological study and biochemical analysis including the living cell number, emulsifying capacity, lipase activity, residual oil, COD and BOD, show that the yeast strain has a strong capability to adapt the highly oily environment, grows fast, and rapidly degrades lipids and organic substances. The strain itself has significant contribution for emulsification, especially with its large amount of living cells, and at the same time synthesizes 21.5 g/L bioemulsifiers which has strong emulsifying potentials. Therefore, the treating system rapidly transforms into a homogeneous situation of complete emulsification. The strain also produces effective extracellular lipase with an activity of 68.3 U/mL, and the enzyme is stable and could perform the hydrolysis function for a long period in the treating environment. The microbial technique of biodegradation of the wastewater with high lipid concentration could stably undergo at high operation loads of lipid. Through the carry-out of biodegrading treatment with the Candida strain, the contents of lipids, COD and BOD are quickly removed out for more than 95%.

关 键 词：高含油废水 生物降解 假丝酵母 性能分析 

分 类 号：TS228[轻工技术与工程—粮食、油脂及植物蛋白工程] X792[轻工技术与工程—食品科学与工程]