异鼠李素对人肺癌细胞抑制作用的观察  

A Study on Inhibitory Effect of Isorhmnetin on Human Lung Cancer Cell

作　　者：杨春蕾[1] 王正荣[2] 张敏[3] 陶大昌[1] 

机构地区：[1]四川大学生命科学院医学细胞生物学教研室,四川成都610041 [2]四川大学基础与法医学院生物医学工程教研室,四川成都610041 [3]四川大学基础与法医学院同位素室,四川成都610041

出　　处：《航天医学与医学工程》2004年第4期261-265,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助(399970275)

摘　　要：目的研究异鼠李素对人肺癌YTLMC-90细胞的抑制作用并探讨其作用机制。方法采用细胞生长、克隆形成和3H-TdR掺入实验,流式细胞术等方法研究异鼠李素对YTLMC-90细胞增殖和凋亡的作用及其分子机制。结果异鼠李素抑制YTLMC-90细胞的生长和增殖,诱导凋亡,使bcl-2,c-myc、H-ras癌基因表达下降,增强bax、c-fos基因表达。结论异鼠李素可能通过下调bcl-2、c-myc、H-ras基因的表达,增强bax、c-fos基因表达来达到抑制YTLMC-90细胞增殖和生长,诱导凋亡和分化。Objective To study the inhibitory effect of isorhmnetin on human lung tumor YTLMC-90 cells and to elucidate its molecular mechanisms. Method Inhibition growth of YTLMC-90 cells was measured by growth curve , clone formation test,3H-thymidine uptake assay ; cell cycle and the expression of genes were analyzed by flow cytometry. Result Growth of YTLMC-90 cells was inhibited evidently after treatment by isorhmnetin and was induced apoptosis. Furthermore, it decreased the expression of bcl-2, c-myc, H-ras oncogene and increased the expression of bax and c-fos. Conclusion Isorhmnetin inhibited cell proliferation and induced apoptosis of YTLMC-90 cells, which might be associated with down-regulation of the expression of bcl-2, c-myc and H-ras oncogenes and up-regulation of expression of bax and c-fos genes.

关 键 词：异鼠李素 肺癌 凋亡 原癌基因 

分 类 号：R36[医药卫生—病理学]