抑颤汤治疗帕金森病的作用机制研究  

Study on the mechanism of therapeutic effect of "Yichantang" in the treatment of Parkinson disease

作　　者：窦永起[1] 杨明会[1] 刘毅[1] 王炜[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《解放军医学杂志》2004年第8期674-676,共3页Medical Journal of Chinese People's Liberation Army

基　　金：军队"十五"医药卫生科研基金 (编号 0 1MA1 1 6)资助课题

摘　　要：目的　探讨抑颤汤治疗帕金森病 (PD)的作用机制。方法　运用 6 羟基多巴诱发法建立大鼠PD模型 ,通过随机分组 ,观察抑颤汤对PD大鼠行为特征的影响 ;采用脑组织液微透析技术 ,用高效液相色谱法测定各组大鼠脑组织细胞外液中儿茶酚胺类物质含量的变化 ;用放免法测定各组大鼠双侧脑组织M、DA受体数量及亲和力的变化 ;用光镜和电镜观察各组大鼠脑黑质细胞形态学变化。结果　抑颤汤能明显改善PD模型大鼠的旋转行为(P <0 0 1) ;抑颤汤组PD大鼠损毁侧脑组织透析液中 3,4 二羟基苯酸 (DOPAC)、高香草酸(HVA)、多巴胺 (DA)、5 羟色胺(5 HT)水平以及脑组织DA和M受体最大结合量 (Bmax)和亲和常数 (KD)均明显高于生理盐水组 (P <0 0 5 )。病理学观察见抑颤汤组大鼠受损侧脑黑质神经细胞数量明显多于生理盐水组 ,且神经元体积较饱满 ,结构较清晰 ,细胞内高尔基体、线粒体等接近正常。结论　抑颤汤能减轻PD模型大鼠脑黑质细胞的受损程度 ,促进其修复 ,改善黑质纹状体系统的分泌功能 ,提高脑组织中内源性儿茶酚胺类物质的含量 ,刺激PD模型大鼠受体系统 ,使DA、M受体的亲和力和敏感性提高 ,从而改善PD大鼠的旋转行为。Objective To explore the mechanism of therapeutic effect of Yichantang in the treatment of Parkinson′s disease (PD). Methods A rat model of PD was replicated. The experimental animals were divided into a normal control group,a normal saline control group and a Yichantang group. Changes in the rats' behavior pattern was obrerved. Using the technique of intracerebral dialysis,the content of catecholamine like substances in the nigral extracellular fluid(NECF)was measured by means of high performance liquid chromatography(HPLC). Changes in the affinity and quantity of M and DA receptors were determined too. Results Yichantang markedly improved the rats' rotation behavior,and enhanced the Bmax and KD of DA and M receptors in the brain tissues on the side of lesion. The levels of DOPAC,HVA,DA and 5-HT in the NECF of the side of lesion in the Yichantang group were significantly higher than that of the normal saline group ( P <0.05). Besides,the number of substance nigra cells was significantly larger in Yichantang group than that in the normal saline group,and the volume and structure of the neurons,intracellular Golgi complex and mitochondria were similar to the normal. Conclusion Yichantang can facilitate the repair of the injured substantia nigra neurons,increase the secretion of DA from striatum-substantia nigra system, and increase the affinity and sensitivity of DA and M receptors by stimulating the receptor system of the PD rats. All these effects may contribute to the therapeutic effect of Yichantang.

关 键 词：帕金森病 作用机制 中医疗法 抑颤汤 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R256.46[医药卫生—临床医学]