P_(53) Codon72多态性与新疆维吾尔族、汉族宫颈癌发生的相关性研究  

Correlation Between P_(53) Codon72 Polymorphism and Cervical Carcinoma among Uigur and Han People

作　　者：王晓凌[1] 潘晓琳[1] 高志斌[1] 郑兴征[1] 李洪安[1] 蒋金芳[1] 

机构地区：[1]石河子大学医学院基础医学系,新疆石河子832002

出　　处：《石河子大学学报（自然科学版）》2004年第4期340-344,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金资助项目(30260036)

摘　　要：采用限制性酶切片段多态性分析技术检测73例维吾尔族宫颈癌组织及26例正常宫颈组织、38例汉族宫颈癌及34例正常宫颈石蜡包埋组织中P53基因多态性的分布,探讨P53基因第四外显子第72位密码子多态性与新疆维吾尔族、汉族人群宫颈癌发生的遗传易感性方面的关系,及其分布是否存在种族差异。结果发现,P53的3种基因型Arg Arg、Pro Pro、Pro Arg在维吾尔族宫颈癌中所占比例分别是47.9%、20.6%、31.5%,相对于对照组的30.8%、19.2%、50.0%,总构成比未见显著性差异(χ2=3.12,P>0.05),但可见Arg Arg所占比例高于对照组,且高于其它二型;在汉族宫颈癌中所占比例分别为21.0%、39.5%、39.5%,与对照组38.2%、14.7%、47.1%相比,存在显著性差异(χ2=6.01,P<0.05),Pro Pro在宫颈癌中所占比例高于对照组。结果表明,P53Pro Pro基因型可能是新疆汉族人群宫颈癌发生的一个主要遗传易感因素,P53codon72多态性是否与新疆维吾尔族宫颈癌的发生相关,尚需进一步研究。In order to study the associationship between P_(53) exon 4 codon72 polymorphism and cervical carcinoma in Uigur and Han patients in Xinjiang,China,and whether there exists the difference about the proportions of P_(53) codon72 polymorphism among nations or not,we used semi-nested PCR followed by confirmation with BstUI restriction enzyme digestion,the P_(53) arg/pro sequence was determined in formalin-fixed,paraffin-embedded biopsy samples from 73 Uigur cervical carcinoma samples. Using PCR and BstUI digestion,26 Uigur normal control,38 Han cervical carcinoma and 34 normal control were detected. The results showed that the proportions of P_(53) genotypes,Arg/Arg?Pro/Pro?Pro/Arg were 47.9%,20.6%,31.5% in Uigur cervical carcinoma,and 30.8%,19.2%,50.0% in control respectively. In statistical analysis,the difference of omni-constituent ratio was not significant(χ~2=3.12,P>0.05),but arginine allele had high ratio.In Han cervical carcinoma cases,the distribution of P_(53) genotypes were 21.0%,39.5%,39.5%.Compared with control gourp 38.2%,14.7%,47.1%,the discrepancy of omni-constituent ratio was significant(χ~2=6.01,(P<0.05)),there was an overrepresentation of homozygous proline compared with the control and other types. We assumed that Homozygous for the proline of the P_(53) and not arginine homozygosity,could be a risk factor affecting host susceptibility to the development of cervical carcinoma among Han women.A further study was needed about correlation between P_(53) polymorphism and cervical carcinoma by the way of genetics in Uigur people.

关 键 词：宫颈癌 P53 多态性 维吾尔族 

分 类 号：R737.3[医药卫生—肿瘤]