大籽蒿化学成分分析及抗癌活性研究  

Studies on the chemical constituents of Artemisia sieversiana and their anticancer activities

作　　者：张琪[1] 郭国宁[2] 苗瑞东[1] 陈能煜[3] 王勤[1] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]华中科技大学生命科学学院,湖北武汉430074 [3]兰州大学化学化工学院,甘肃兰州730000

出　　处：《兰州大学学报（自然科学版）》2004年第4期68-71,共4页Journal of Lanzhou University(Natural Sciences)

基　　金：甘肃省科技攻关资助项目(GS022-A43-136)

摘　　要：将大籽蒿(Artemisia sieversiana Ehrh.ex Willd.)地上部分乙酸乙酯提取物用硅胶柱层析分离和纯化,通过波谱方法鉴定化合物,用MTT法、单细胞电泳进行体外抗肿瘤活性研究.鉴定出4种化合物:洋艾素;Achillin;5,7,4’-三羟基-3’,5'-二甲氧基黄酮;3,5-二羟基-6,7,3’,4’-四甲氧基黄酮.MTT法结果表明洋艾素和Achillin对人肝癌细胞SMMC-7721的生长有较明显的抑制作用,且具有一定的时间和浓度依赖性。单细胞电泳显示对SMMC-7721细胞的DNA有损伤作用.所以洋艾素和Achillin对SMMC-7721细胞株的抑制活性与其DNA损伤有关.Four compounds were isolated from the methanolic extract of the aerial parts of Artemisia sieversiana, and identified by means of chemical and modern spectral methods. Cell survival rate was determined by MTT, and DNA damage was estimated using single cell gel electrophoresis (comet assay). Absinthin and achillin showed inhibitory effects on SMMC-7721 cell lines in vitro and therefore, they might have certain anticancer activities by damaging DNA.

关 键 词：大籽蒿 化学成分 抗癌活性 

分 类 号：Q629.6[生物学—生物物理学]