昆明鼠对猪轮状病毒核酸免疫的抗体应答  

The antibody response of Kunming mice against the nucleic acid immunization of procine rotavirus

作　　者：师东方[1] 贾永清[1] 李一经[1] 王君伟[1] 马波[1] 刘宝全[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2004年第4期446-448,共3页Journal of Northeast Agricultural University

基　　金：黑龙江省"九五"及"十五"科技攻关计划项目。编号(G99B8-1-1;GC0113510)。

摘　　要：将昆明鼠随机分为A、B两组,分别肌肉注射含有猪轮状病毒(RV)JL94株VP7基因的重组真核表达质粒pcDNA-VP7和真核表达载体pcDNA3.1(+),每只鼠100μg·(100μL)-1,共免疫3次,每次间隔2周。首次免疫第0,14,21,28,35,39,47,54天采血分离血清用ELISA法检测抗体动态变化。A组小鼠血清在首免后第14天即可检出阳性抗体(P/N≥2.0)。The Kunming mice used for nucleic acid immunization were divided into two groups. Group A was injected with 100 μg of the recombinant plasmid, pcDNA-VP7, which contained VP7 gene of the native JL94 isolate of porcine rotavirus, group B with 100 μg of eukaryotic expression plasmid pcDNA3.1(+)in 100 μL volume via femoral quadriceps intramuscular route, respectively, and the mice in each group were injected three times totally. After primary injection, all mice received booster injection two times with the same dosage and via the same route of primary injection at interval of two weeks. Blood samples collected from mice at 0 d before primary injection and those collected from mice of each group at 14, 21, 28, 35, 39, 47, 54 d, were used for detecting antibody levels by ELISA. The results showed that the antibodies to RV could be detected in the serum of mice from group A from the beginning of 14 days of post-immunization(PI).

关 键 词：轮状病毒 VP7基因 核酸免疫 抗体 

分 类 号：S865.13[农业科学—野生动物驯养] S828[农业科学—畜牧兽医]