新型氨基酸注射液对创伤大鼠DNA损伤作用的研究  

EFFECT OF THE NEW AMINO ACID INJECTION ON DNA DAMAGE IN TRAUMATIZED RATS

作　　者：庞伟[1] 蒋与刚[1] 徐静[1] 杨继军[1] 韦京豫[1] 郭长江[1] 徐琪寿[2] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]军事医学科学院放射医学研究所

出　　处：《解放军预防医学杂志》2004年第4期249-251,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：天津市科技攻关培育项目 (NO .0 43 10 0 2 11)

摘　　要：目的以国产创伤用 17种和 18种氨基酸注射液为对照 ,观察 2 0种新型氨基酸注射液 (含有谷氨酰胺、精氨酸、牛磺酸等 )对创伤大鼠外周血淋巴细胞DNA损伤及血浆丙二醛(MDA)浓度的影响。方法雄性Wistar大鼠 (体重 180～ 2 0 0g) ,行创伤和中心静脉插管手术后随机分为创伤对照组、17AA组、18AA组和 2 0AA组 ,每组 8只。分别输以生理盐水、2 .6 % 17种氨基酸注射液、2 .6 % 18种氨基酸注射液和 2 .6 % 2 0种新型氨基酸注射液。 7d后经眼眶采血 ,分离其外周血淋巴细胞 ,利用单细胞凝胶电泳技术检测创伤大鼠外周血淋巴细胞DNA损伤 ,同时测定血浆MDA浓度。结果 2 0AA组大鼠外周血淋巴细胞DNA移动距离〔(16 .92± 4 .2 4 ) μm〕和尾长〔(8.31± 1.4 9) μm〕分别明显低于 17AA组〔(2 8.0 0± 5 .2 7) μm、(13.4 7± 1.94 ) μm〕和 18AA组〔(2 5 .2 5± 4 .5 7) μm、(13.32± 1.75 ) μm〕 ;而其血浆MDA值〔(9.2 0± 1.0 5 ) μmol·L- 1〕较 17AA组〔(15 .88± 1.4 0 ) μmol·L- 1〕和 18AA组〔(19.95± 8.6 5 ) μmol·L- 1〕下降 (P <0 .0 1,P <0 .0 5 )。结论新型 2 0种氨基酸注射液可减轻创伤大鼠外周血淋巴细胞DNA损伤并抑制其脂质过氧化的发生。Objective To investigate the effect of 20-crystalline amino acid (20AA) solution on lymphocyte DNA damage in traumatized rats. Methods Thirty-two male Wistar rats were randomly divided into four groups after trauma operation,i.e.traumatized control group(infused saline),17AA group,18AA group and 20AA group.After seven day infusion,blood lymphocytes were separated,and DNA damage was detected and analysed by single cell gel electrophoresis (SCGE ).MDA concentration in plasma was also measured. Results Comet cells appeared in every traumatized group; DNA shift distance〔(16.92±4.24)μm〕 and tail length 〔(8.31± 1.49)μm〕 in 20AA group were significantly lower than those in 17AA group 〔(28.00±5.27)μm and (13.47± 1.94)μm,respectively P<0.01〕, and 18AA group 〔(25.25±4.57)μm and (13.32± 1.75)μm,respectively P<0.01〕.Plasma MDA level in 20AA group 〔(9.20±1.05)μmol·L -1〕 was obviously lower than that in 17AA group 〔(15.88±1.40)μmol·L -1, P<0.01〕 and 18AA group 〔(19.95±8.65)μmol·L -1, P<0.05〕. Conclusion The new 20 Amino acid solution can reduce DNA damage and inhibit lipid peroxidation in traumatized rats.

关 键 词：氨基酸注射液 创伤 DNA损伤 单细胞凝胶电泳 

分 类 号：R151.2[医药卫生—营养与食品卫生学]