脑溢安对新生大鼠海马神经干细胞缺氧损伤及p38MAPK活性的影响  

Effects of nao yi-an on p38MAPK activity in hippocampal neural stem cells of newborn rats after hypoxia injury?

作　　者：肖岚[1] 黎杏群[1] 刘柏炎[1] 罗杰坤[1] 黄菊芳[2] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008 [2]中南大学湘雅医学院神经生物研究所,湖南长沙410008

出　　处：《中国现代医学杂志》2004年第16期67-70,74,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金资助(编号:30171192)

摘　　要：目的探讨中药脑溢安颗粒(简称脑溢安)对神经干细胞缺氧损伤的影响及其保护作用机制.方法体外培养神经干细胞来自新生3～5 d SD大鼠海马组织,造缺氧损伤模型,用原位末端标记法(TUNEL)进行细胞凋亡检测,应用免疫共沉淀、激酶反应及Western blot技术研究脑溢安对缺氧损伤神经干细胞内p38丝裂原活化蛋白激酶(p38 Mitogen-activated protein kinase,p38MAPK)活性的影响.结果培养神经干细胞缺氧损伤后发生凋亡,脑溢安能促进缺氧损伤神经干细胞存活及减少神经干细胞凋亡;缺氧损伤后神经干细胞内p38MAPK活性增强,脑溢安血清组p38MAPK活性显著低于模型组和正常血清组(P＜0.01).结论脑溢安能保护缺氧损伤神经干细胞,抑制缺氧损伤激活的p38MAPK信号转导通路可能为其保护作用机制之一.Objective: To explore the effects and protective mechanisms of the traditional Chinese medicine complex nao yi-an granule (NYA) on the neural stem cells (NSCs) after hypoxia injury. Methods: The cultured hippocampal neural stem cells (NSCs) were taken from the newborn Sprague-Dawley (SD) rats at 3~5 days, the NSCs were cultured at 37℃ in 95%N2 and 5%CO2 for 6 h to make the cells hypoxia injuried; The apoptosis was studied with the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, p38 MAPK activity in the NSCs was analysed after hypoxia injury with immunoprecipitation/kinase assay/Western blot. Results: Apoptosis was induced by hypoxia injury in the NSCs, NYA serum can promote the NSCs survival and inhibit the apoptosis; The activation of p38 was increased after hypoxia injury, the p38 activity in NYA serum group was significantly lower than the model group and normal-serum group (P<0.01). Conclusions: NYA serum can protect NSCs after hypoxia injury, inhibiting the hypoxia-stimulated p38MAPK signaling transduction pathway may be one of the neuroprotective mechanisms.

关 键 词：大鼠 神经干细胞 缺氧损伤 P38MAPK 中药脑溢安i…^ ㈧…H一. 

分 类 号：R-332[医药卫生]