溃疡性结肠炎肠粘膜细胞中糖皮质激素受体α和β的表达  

Study on Glucocorticoid Receptor Alpha and Beta in Colonic Mucosal Cell of Patients with Ulcerative Colitis

作　　者：张虎[1] 欧阳钦[1] 温忠慧[1] 刘卫平[2] 陈代云[2] 李俸媛[2] 

机构地区：[1]四川大学华西医院消化科,成都610041 [2]四川大学华西医院病理科

出　　处：《四川大学学报（医学版）》2004年第5期630-633,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　研究溃疡性结肠炎患者肠粘膜细胞中糖皮质激素受体 α、β(GRα、GRβ)表达与糖皮质激素治疗反应以及炎症程度之间的关系。方法　采用免疫组织化学法检测肠粘膜细胞中 GRα、GRβ表达强度。比较激素抵抗、激素敏感及正常对照 3组间以及炎症程度各组间 GRα、GRβ表达差异。结果　肠粘膜组织切片中 GRα、GRβ的表达强度在抵抗与敏感组之间有显著性差异 (P<0 .0 5 ) ,在激素敏感组与正常对照组间无显著差异 ,且与炎症程度无关。结论　糖皮质激素受体 GRα、GRβ在决定患者对激素治疗的反应中起着重要的作用 ,其中 GRβ可能对 GRα起优势负性抑制作用 ,检测患者肠粘膜细胞中 GRα、GRβ的表达可预测患者对激素治疗的反应 。Objective To investigate the relationship of the expression of GRα and GRβ in the colonic mucosal cell of patients with ulcerative colitis to the efficacy of glucocorticoid (GC) therapy and the intensity of inflammation. Methods GRα expression and GRβ expression in the colonic mucosal specimens were assessed by means of immunohistochemistry. Then comparative analyses were made on the GRα and GRβ expression between the GC resistant group and GC sensitive group at various levels of inflammation. A normal response group was observed for comparison. Results The staining intensities of both GRα and GRβ in colonic mucosal specimens showed significant differences between the GC resistant group, GC sensitive group and normal response group. No association between the expression of GRα and GRβ and the degree of inflammation was found. Conclusion These findings suggest that both GRα and GRβ play an important role in the mechanism of the action of GC, and GRβ functions as a dominant negative inhibitor of GRα in there. The expression of GRα and GRβ in the colonic mucosal cell of patients with ulcerative colitis may serve as predictors of glucocorticoid response, but cannot be used as the markers of the severity of inflammation.

关 键 词：溃疡性结肠炎 肠粘膜细胞 糖皮质激素受体α亚型 糖皮质激素受体β亚型 

分 类 号：R574.62[医药卫生—消化系统]