鸡白细胞介素2在毕赤酵母细胞中的表达  

The expression of chicken interleukin 2 in Pichia yeast

作　　者：许健[1] 樊拥军[1] 李龙[1] 万旺军[1] 李建荣[1] 于涟[2] 

机构地区：[1]浙江大学生物医学工程系 [2]浙江大学动物预防医学研究所,浙江杭州310029

出　　处：《西安交通大学学报（医学版）》2004年第4期339-342,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：浙江省重点科技项目 (No .0 1 1 1 0 2 4 65);浙江省自然科学基金重大项目 (No .ZA0 1 0 5)

摘　　要：目的　利用Pichia酵母真核细胞表达系统表达重组鸡白细胞介素 2 (IL 2 ) ,为大量制备鸡IL 2奠定基础。方法　将ConA激活的鸡脾脏T淋巴细胞中扩增得到的我国地方品种萧山鸡IL 2基因 ,克隆入pPIC9载体中构建重组质粒。将重组质粒电转化毕赤酵母GS115感受态细胞 ,甲醇诱导蛋白质表达。结果　SDS PAGE和Westernblot均在 30ku处检测到特异条带。结论　表明重组鸡ILObjective To express recombinant chicken interleukin 2 (IL-2) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL-2. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL-2 gene was amplified and cloned into pPIC9. The pPIC9-IL-2 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Results The protein was examined with SDS-PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL-2 has been expressed in Pichia yeast.

关 键 词：鸡IL-2 pPIC9 毕赤酵母 免疫增强剂 

分 类 号：Q78[生物学—分子生物学]