恒磁场对内皮细胞分泌和表达细胞间黏附分子-1及与单核细胞黏附的抑制作用  

Inhibitory effects of constant magnetic field on secretion of intercellular adhesion molecule-1 by endothelial cells and their adhesion with monocytes

作　　者：程何祥[1] 周廉[1] 徐可为[2] 贾国良[3] 王海昌[3] 张荣庆[3] 牛金龙[1] 李争显[1] 

机构地区：[1]西北有色金属研究院生物中心,陕西西安710016 [2]西安交通大学材料学院,陕西西安710049 [3]第四军医大学西京医院,陕西西安710032

出　　处：《西安交通大学学报（医学版）》2004年第4期353-355,358,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助 (No .30 2 70 396)

摘　　要：目的　观察不同强度恒磁场对氧化修饰低密度脂蛋白 (OX LDL)作用下人脐静脉内皮细胞 (HUVEC)分泌和表达细胞间黏附分子 1(ICAM 1)及HUVEC与人单核细胞株THP 1黏附的影响。方法　采用体外培养第 3代的HUVEC ,实验分为 6组 ,即对照组、OX LDL(10 0mg·L-1)组及OX LDL +不同磁感应强度 (1、5、10、2 0Gs)的恒磁场组。各组细胞于培养及恒磁场作用 2 4h后收集标本 ,用酶联免疫吸附试验 (ELISA)检测HUVEC分泌ICAM 1的量 ,免疫细胞化学检测ICAM 1的表达 ,计数法观察HUVEC与THP 1的黏附率。结果　OX LDL 10 0mg·L-1刺激细胞 2 4h,ICAM 1的分泌和表达显著增高(P <0 .0 5vs.对照组 ) ;而 1、5、10和 2 0Gs恒磁场组细胞ICAM 1的分泌和表达显著低于OX LDL组 (P <0 .0 5 )。OX LDL10 0mg·L-1与HUVEC孵育 2 4h后 ,HUVEC与THP 1的黏附率显著增加 (P <0 .0 5vs.对照组 ) ;而 1、5、10和 2 0Gs恒磁场组HUVEC与THP 1的黏附率显著低于OX LDL组 (P <0 .0 5 )。结论　 1～ 2 0Gs的恒磁场可拮抗OX LDL的作用 ,抑制HUVEC分泌和表达ICAMObjective To investigate the effects of constant magnetic field on secretion and expression of intercellular adhesion molecule-1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) and their adhesion with monocytes (THP-1) induced by oxidized low-density lipoprotein (OX-LDL). Methods The third passage of cultured HUVECs were used. The cells were divided into six groups: control group, OX-LDL group, and OX-LDL with one, five, ten or 20 gausses of constant magnetic field groups. Samples were collected 24 hours later. Secretion of ICAM-1 was detected by ELISA, and expression of ICAM-1 was detected by immunocytochemistry. Adhesion rates between HUVEC and THP-1 was measured by counting method. Results Secretion of ICAM-1 significantly increased 24 hours after intervention by OX-LDL of 100 mg·L -1 (P< 0.05 vs. control). However, in one, five, ten and 20 gausses groups, secretion and expression of ICAM-1 significantly decreased compared with those of OX-LDL group (P< 0.05). The adhesion rates between HUVEC and THP-1 significantly increased 24 hours after incubation of HUVEC with OX-LDL (P< 0.05 vs. control). On the other hand, in one, five, ten and 20 gausses groups, adhesion rates between HUVEC and THP-1 significantly decreased compared with those of the OX-LDL group (P< 0.05). Conclusion One gauss to 20 gausses of constant magnetic fields can intensity-dependently antagonize effects of OX-LDL on HUVEC by decreasing secretion and expression of ICAM-1 by HUVEC and decreasing adhesion rates between HUVEC and THP-1.

关 键 词：恒磁场 内皮细胞 单核细胞 细胞间黏附分子一1 氧化修饰低密度脂蛋白 

分 类 号：R363[医药卫生—病理学]