二乙酰去水卫矛醇联合p53基因转染对肝癌细胞生长的影响  

Effect of 1,2:5,6-dianhydro-3,4-diacetylgalactitol and p53 gene transfection for the growth of hepatocellular carcinoma

作　　者：赖祥进[1] 曹林枝[2] 周瑛 蓝秀万[1] 廖志红[1] 周素芳[1] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,广西南宁530021 [2]右江民族医学院生物化学教研室,广西百色533000 [3]广西中西医药研究所,广西南宁530021

出　　处：《中国癌症杂志》2004年第4期309-312,共4页China Oncology

基　　金：广西自然科学基金 (桂科自 9912 0 3 5 )资助

摘　　要：目的 :观察野生型p5 3基因转染联合二乙酰去水卫矛醇 (1,2∶5 ,6 dianhydro 3,4 diacetylgalactitol,DADAG)对肝癌细胞生长的影响。方法 :将克隆有野生型p5 3基因的pUHD10 3质粒 ,通过脂质体 (lipofectamine)介导转染人肝癌细胞 ,同时 ,在培养液中加入DADAG ,用四甲基偶氮唑 (MTT)法分析细胞的生长情况。结果 :人肝癌细胞HLE转染野生型p5 3基因后 ,其生长作用明显受到抑制 ,细胞生长抑制率在第 4天可达 6 7.6 8%。如果HLE细胞中只加入含DADAG的培养液后 ,其细胞生长也受到一定的抑制 ,生长抑制率最高可达到 5 3.0 9%。肝癌细胞HLE转染野生型p5 3基因后联合应用DADAG时 ,细胞的生长抑制率在第 4天可达到 85 .37% ,实验结果显示DADAG联合野生型p5 3基因增强对肝癌细胞的生长抑制作用。结论 :DADAG能加强野生型p5 3基因对肝癌细胞的生长抑制作用。Purpose:To investigate the effect of p53 gene transfection and 1,2∶5,6-dianhydro-3,4-diacetylgalactitol (DADAG) on the growth of hepatocellular carcinoma(HCC). Methods:The HCC line HLE that were transfected with plasmid pUHD10-3 recombined with wt-p53 gene by lipofectamine-mediated were cultured in medium containing DADAG. 3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid(MTT) technique was used to analyse the growth of HCC cells. Results:The growth of HCC cell line HLE were inhibited after being transfected with wt-p53 gene,and 67.68% of the cells were inhibited on the fourth day. 53.09% of HCC cell line HLE were inhibited when they were exposed to DADAG. When the HCC cell line HLE transfected with wt-p53 gene were exposed to DADAG,85.37% of the cells were inhibited. Conclusions:The inhibition efficiency of HCC was enhanced by the combination of DADAG and wt-p53 gene transfection.

关 键 词：P53基因 肝癌 二乙酰去水卫矛醇 

分 类 号：R735.7[医药卫生—肿瘤] Q785[医药卫生—临床医学]