乳腺癌组织中端粒酶活性的定量检测及其与临床病理的关系  

Quantitative Detection of Telomerase Activity and Its Association with Clinico pathological Characteristics in Breast Cancer

作　　者：何国平[1] 税青林[2] 黄燕[2] 张思仲[1] 余红[2] 

机构地区：[1]四川大学华西医院医学遗传学研究室人类疾病基因组学研究室人类疾病生物治疗教育部重点实验室,四川成都610041 [2]泸州医学院医学生物学与遗传学教研室,四川泸州646000

出　　处：《癌症》2004年第9期1041-1046,共6页Chinese Journal of Cancer

基　　金：四川省教委重点项目基金资助[川教计(1999)164号]~~

摘　　要：背景与目的:端粒酶是一种在细胞永生化及癌症发生过程中起重要作用的核蛋白酶。最近关于乳腺癌中端粒酶活性与预后因素间的关系,因研究方法的差异而呈现出不一致的报道。本研究旨在建立一种可行的银染端粒酶定量检测法,以探讨乳腺癌中端粒酶活性与临床病理学预后因素间的关系。方法:运用银染端粒重复序列扩增法(SS-TRAP)检测了52例新鲜乳腺癌及其癌旁组织,32例乳腺良性病变和14例正常乳腺组织中端粒酶的活性表达,对结果予以定量并结合临床资料进行分析。结果:乳腺癌、癌旁组织、良性病变及正常乳腺组织中端粒酶阳性率分别为90.38%(47/52)、28.85%(15/52)、31.25%(10/32)、0(0/14)。端粒酶分别为36.91±15.35、8.27±4.37、14.10±5.28、0(TPG单位),单因素方差分析结果显示,乳腺癌组端粒酶活性水平明显高于其他3组(P值均<0.01)。Logistic回归分析结果表明,乳腺癌中端粒酶的表达与病理类型、分化程度明显相关(P=0.003及0.004),即随着肿瘤的进展,端粒酶活性亦增加。其中,浸润性非特殊癌端粒酶活性水平明显高于浸润性特殊癌(P<0.05);中、低分化癌端粒酶活性均高于高分化癌(P<0.05),中、低分化癌间无显著性差异(P>0.05)。端粒酶活性表达在患者病程、年龄、绝经状况间均无显著性差异(P>0.05)。BACKGROUND &OBJECTIVE: Telomerase is a ribonucleoprotein enzyme, w hich plays an important role in cell immortalization and carcinogenesis. Recent studies on the association of telomerase activity with prognostic factors of bre ast cancer were controversial due to different methods. This study was to establ ish a feasible assay of quantitative detection of telomerase activity based on s ilver staining, and investigate possible association between telomerase activity and clinicopathological prognostic factors in primary breast cancer. METHODS: H ighly sensitive silver-staining telomeric repeat amplification protocol assay ( SS-TRAP) was used to quantify telomerase activity in 52 frozen human breast can cer samples and their adjacent breast tissues, 32 benign lesions, and 14 normal mammary gland lesions. The association between telomerase activity and clinicopa thological data was analyzed. RESULTS: Telomerase activity was detected in 47 of the 52 breast cancer samples (90.38%), and in 10 of the 32 benign lesions, whe reas no activity was detected in 37 of 52 adjacent nonmalignant breast tissues, and all 14 normal mammary gland tissues. The telomerase activity levels were 36. 91±15.35, 8.27±4.37, 14.10±5.28, 0 (unit: TPG) in breast cancer, adjacent tis sue of cancer, benign lesion, and normal tissue, respectively. The difference of telomerase activity was significant between breast cancer and the other 3 group s by using ANVOA (all P< 0.01). A strong correlation was found between telomeras e activity and pathological category, and differentiation degree by logistic reg ression analysis, i.e. with ongoing tumor progression, telomerase activity appea red to increase in primary breast cancer (P=0.003, and P=0.004).No correlation w as seen between telomerase activity and disease course, age, and menopause statu s of patients (all P >0.05). Telomerase activity level of invasive non-special cancer was higher than that of invasive special cancer(P< 0.05). Telomerase acti vity level of moderately/poorly differentiated c

关 键 词：乳腺肿瘤 端粒末端转移酶 定量分析 

分 类 号：R737.9[医药卫生—肿瘤]