猪心脏脂肪酸结合蛋白基因的克隆和测序分析  

Cloning and Sequencing of Heart Fatty Acid-binding Protein Gene in Porcine

作　　者：陈小波[1] 廖国周[1] 花群义[2] 葛长荣[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南出入境检验检疫局技术中心,云南昆明650228

出　　处：《云南农业大学学报》2004年第4期378-380,共3页Journal of Yunnan Agricultural University

基　　金：云南省十五攻关项目(2001NG40)

摘　　要：根据GeneBank发表的猪心脏脂肪酸结合蛋白(heartfattyacid bindingprotein,H-FABP)基因序列,设计合成一对引物,从杜长大三元杂交猪的腰大肌中提取总RNA,对H-FABP基因进行RT-PCR扩增,产物经琼脂糖电泳分析,呈现一条约124bp的条带,回收纯化后,将其克隆至pMD18-T质粒载体中,然后进行核苷酸序列分析。与GeneBank中报道的H-FABP基因比较后发现,该基因片段与其它的核苷酸的同源性为94%～100%,推导的氨基酸的同源性为100%.Heart fatty acid-binding protein gene isolated from porcine (Duroc×Landrace×Yorkshire) muscle tissue, were amplified by RT-PCR. The product of 0.124 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pDM18-T vector and identified by PCR and sequence. The results demonstrated that nucleotide homology with other published H-FABP genes was between 94%～100%, and deduced amino acid homology was between 100% respectively.

关 键 词：猪 心脏脂肪酸结合蛋白基因 RT-PCR 基因克隆 序列分析 

分 类 号：S828[农业科学—畜牧学] S821.2[农业科学—畜牧兽医]