人牙源性间充质细胞与胶原类材料复合培养的体外实验研究  

A Study on Human Dental Mesenchymal Cells Cultured in 3-D Collagen Materials in Vitro.

作　　者：包柳郁[1] 金岩[1] 史俊南[1] 牛忠英[2] 汪平[1] 赵守亮[1] 郝建军[1] 王捍国[1] 

机构地区：[1]第四军医大学口腔医学院牙体病科,陕西西安710032 [2]解放军第三零六医院

出　　处：《口腔医学研究》2004年第4期337-339,共3页Journal of Oral Science Research

基　　金：国家 8 63计划组织工程重大专项资助项目 (编号 :2 0 0 2AA2 0 5 0 41);国家自然科学基金资助项目 (编号 :3 0 2 70 3 74)

摘　　要：目的 :评价胶原类材料作为牙齿组织工程研究的支架材料的可行性。方法 :将生长因子诱导后的人牙源性间充质细胞与胶原类材料复合后进行体外三维培养 ,观察细胞生长和增殖情况。结果 :细胞在胶原凝胶中生长良好 ,并保持了分化表型。细胞在胶原膜表面伸展充分 ,生长良好 ,增殖活跃 ,细胞表面可见多数分泌颗粒。结论 :胶原类材料适宜牙源性间充质细胞的生长。然而 ,它们坚韧性不高 ,而且无法为矿化组织细胞的生长提供充足的矿物离子环境。因此 。Objective: To evaluate the feasibility of collagen materials as scaffolds for tissue engineering of tooth. Methods: The induced human dental mesenchymal cells were seeded onto collagen materials. After in vitro culture, cell growth and proliferation were determined with HE staining, immunofluorescent staining and SEM. Results:The cells adhered and proliferated well on collagen gel, and kept the differentiated phenotype. The cells also grew well on the collagen membrane, and many secretary particles could be found on the cellular surface. Conclusion: Collagen materials showed good biocompatibility with dental mesenchymal cells. But collagen materials aren't hard enough, and can't provide sufficient mineralization microenvironment for seeding cells. So, Collagen materials should be used as assistant scaffolds for tissue engineering of tooth.

关 键 词：胶原 支架材料 间充质细胞 牙齿 组织工程 

分 类 号：R783.1[医药卫生—口腔医学]