高碘对小鼠抗氧化能力的影响及硒的干预作用  

Influence of high iodine intake on anti-oxidation ability in mice and intervention of selenium

作　　者：杨雪锋[1] 候晓晖[1] 庞红[1] 张旭照[1] 祁振英[1] 于东[1] 孙秀发[1] 

机构地区：[1]华中科技大学同济医学院营养与食品卫生学系,武汉430030

出　　处：《中国公共卫生》2004年第9期1081-1082,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金重点项目 (30 2 30 330 )

摘　　要：目的　研究高碘摄入对小鼠抗氧化能力的影响及硒的干预作用。方法　将 4 0只Balb/c小鼠按体重随机分为 5组 :正常对照组、高碘Ⅰ组 ( 30 0 0 μg/LⅠ )、高碘Ⅰ ( 30 0 0 μg/LⅠ ) +Se组 ( 10 0 0 μg/LSe)、高碘Ⅱ组 ( 5 0 0 0μg/LⅡ )以及高碘Ⅱ ( 5 0 0 0 μg/LⅠ ) +Se组 ( 10 0 0 μg/LSe) ,分别喂饲高碘水或高碘 +硒水以及正常鼠饲料。 3个月后 ,观察甲状腺病理变化 ,测定血清总T4(TT4)、总T3 (TT3 )、游离T3 (FT3 )、肝脏和脑组织的谷胱苷肽过氧化物酶(GSH -Px)活性以及丙二醛 (MDA)水平。结果　高碘组小鼠出现弥漫胶质性甲状腺肿 ;与正常对照组相比 ,2个高碘组的血清TT4水平显著升高 ;而高碘Ⅱ组血清TT3 水平显著降低 ;高碘组肝脏和脑组织GSH -Px活性降低 ,MDA含量升高。而补硒会升高GSH -Px活性 ,其中高碘Ⅰ +Se组GSH -Px活性显著高于高碘Ⅰ组。结论　高碘摄入会降低小鼠体内的抗氧化能力 。Objective To study the influence of high iodine intake on the anti-oxidation ability in mice and intervention of selenium.Methods 40 Balb/c mice were divied into 5 groups according to weight:control group,high iodine group Ⅰ( 3?000?μg/L Ⅰ),hihg iodine Ⅰ(3?000?μg/L Ⅰ)plus selenium(1?000?μg/L Se) group,high iodine group Ⅱ(5?000?μg/L Ⅰ)and high iodine Ⅱ(5?000?μg/L Ⅰ) plus selenium(1?000?μg/L Se) group.The mice were given drinking water including different doses of iodine and selenium.3 months later,related indices were determined.Results The mice in high iodine group showed colloid goiter.Compared with control group,serum TT\-4 level in two high iodine groups increased significantly,serum TT\-3 in high iodine group Ⅱ decreased obviously;In high iodine group,liver and brain GSH-Px activity decreased,MDA content increased,and GSH-Px activity in high iodine Ⅰ plus selenium group was significantly higher than high iodine group Ⅰ.Conclusion High iodine intake may decrease the anti-oxidation ability in mice and selenium supplementation exerted favourable effects on it.

关 键 词：高碘 硒 谷胱苷肽过氧化物酶 丙二醛 

分 类 号：R151.2[医药卫生—营养与食品卫生学]