基于PLC的无糖组培微环境控制系统  

Application of PLC in photoautotrophic plant tissue culture environment control system

作　　者：李传业[1] 滕光辉 曲英华[1] 

机构地区：[1]中国农业大学水利与土木工程学院,北京100083

出　　处：《中国农业大学学报》2004年第4期30-34,共5页Journal of China Agricultural University

基　　金："十五"国家科技攻关资助项目 ( 2 1316 0 0 4 )

摘　　要：为实现组培微环境CO2 增施的长期自动控制 ,解决增施CO2 过程中可能将空气中杂菌引入组培箱的问题 ,设计开发了一套基于PLC的无糖组培微环境控制系统。系统以PLC为控制核心 ,采用定量供给的CO2 增施控制策略 ,可根据设定要求将固定量的高压、高纯度CO2 气体直接供入组培箱 ,并可对组培箱内湿度进行调控。系统调试试验结果表明 ,控制系统能将光期组培箱内CO2 摩尔分数和相对湿度分别控制在 70 0～ 90 0 μmol·mol-1和80 %～ 92 %的目标范围内 。In order to realize CO 2 enrichment automatic control over a long period of time and overcome the drawback of common plant tissue culture environment control system that uses buffer set, a photoautotrophic plant tissue culture environment control system was designed. The core of this system is PLC. The control system controls the discharge of CO 2 over a long period of time by fixed quantity supply. The results indicated that this environment control system could be operated normally and reliably. The CO 2 concentration in the setting scope of 700—900?μmol·mol -1 and relative humidity in setting scope of 80 %—92% could be kept.

关 键 词：植物组织培养 无糖培养 PLC 环境调控 

分 类 号：S604.3[农业科学—园艺学] S625.51