千金子甲醇提取物抗肿瘤作用的实验研究  

Experimental Study on the Anti-tumor Effect of Methanolic Extractive from Euphorbia Lathyris L.in Vitro and in Vivo

作　　者：黄晓桃[1] 黄光英[1] 薛存宽[2] 孔彩霞[2] 何学斌[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030 [2]华中科技大学同济医学院附属梨园医院老年医学研究所

出　　处：《肿瘤防治研究》2004年第9期556-558,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　观察千金子甲醇提取物体内、外抗肿瘤活性及其量效关系。方法　体外药效试验用MTT法 ,体内用小鼠移植性肿瘤 ,观察千金子甲醇提取物的抑瘤活性。结果　千金子甲醇提取物体外对人宫颈癌细胞 (Hela)、人红白血病细胞 (K562 )、人单核细胞性白血病细胞 (U93 7)、人急性淋巴细胞性白血病细胞 (HL60 )和人肝癌细胞 (HepG2 )的IC50 值分别为 15 .5 μg/ml、13.1μg/ml、10 .5 μg/ml、17.5 μg/ml、2 9 .6 μg/ml;体内对小鼠移植性肿瘤细胞株也显示出较显著抑制作用。 结论　千金子甲醇提取物体外对Hela、K562 、U93 7、HL60 、HepG2 有明显的细胞毒活性 ,体内对小鼠肉瘤 180 (S180 )和艾氏腹水癌 (EAC)Objective To study the antitumor activity of methanolic extractive from Euphorbia Lathyris L. both in vitro and in vivo, and further evaluate its does-effect relationship.Methods In vitro and in vivo study on the pharmacological activity of methanolic extractive from Euphorbia Lathyris L. were performed by using MTT assay and mice transplant tumor model respectively. Results IC (50) numerical value of Hela cells、K (562) cells、U (937) cells、HL (60) cells and HepG 2 cells were (15.5)μg/ml、(13.1)μg/ml、(10.5)μg/ml、(17.5)μg/ml、(29.6)μg/ml in vitro respectively, in addition methanolic extractive from Euphorbia Lathyris L. demonstrated antitumor activity in vivo.Conclusion Methanolic extractive from Euphorbia lathyris L. has not only obvious inhibition effect on the human tumor cell strains such as Hela cells、U (937) cells in vitro, but also demonstrated antitumor effect on mice transplant tumors such as S (180)、EAC in vivo.

关 键 词：千金子甲醇提取物 MTT法 人癌细胞株 肉瘤180 艾氏腹水癌 抗肿瘤 

分 类 号：R73-36[医药卫生—肿瘤]