转染人pIgR的小鼠鼻咽上皮细胞在EBV感染前后基因表达谱差异  

Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection

作　　者：王爽[1] 吕丽春[1] 李虹[1] 江培洲[1] 姚开泰[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515

出　　处：《第一军医大学学报》2004年第9期1001-1005,共5页Journal of First Military Medical University

基　　金：广东省"十五"社会发展攻关专项基金(2001A1080201);全军医药卫生科研基金(01Z048)~~

摘　　要：目的应用cDNA阵列技术研究转染人多聚免疫球蛋白受体(hpIgR)的二亚硝基哌嗪(n, n'-dinitrosoperazine, DNP)转化的小鼠鼻咽上皮(TMNE)细胞系在EBV感染前后基因表达谱差异,探索EBV在鼻咽癌发病过程中的作用及鼻咽癌的发病机制。方法提取转染hpIgR 的TMNE细胞在EBV感染前后的总RNA,逆转录成α-32P-dATP标记的cDNA探针,与AtlasTM mouse cancer array 1.2 阵列膜进行杂交。利用AtlasImageTM 软件分析基因表达差异。结果共有25个基因表达水平发生了改变,有23个基因表达上调,2个基因表达下调。结论EBV感染可使转染细胞的多个基因表达发生改变,这些基因可能与鼻咽癌的发生、发展有关。Objective To study the gene expression profile of human polymeric immunoglobulin receptor gene (hpIgR)-transfected mouse nasopharyngeal epithelial cells transformed with n, n'-dinitrosoperazine (TMNE) before and after EBV infection using cDNA array and investigate the role of Epstein-Barr virus (EBV) infection in the tumorigenesis of nasopharyngeal carcinoma (NPC). Methods The total RNAs of hpIgR-transfected TMNE cells before and after EBV infection were extracted, reversely transcribed, and labeled with α -32P-dATP. The cDNA probes were hybridized to the AtlasTM mouse cancer array 1.2, and the signals analyzed by AtlasImageTM software. Results Twenty-five genes differentially expressed in cells before and after EBV infection, including 23 up-regulated genes and 2 down-regulated genes. Conclusions The gene expression profile of hpIgR-transfected TMNE cells may change after EBV infection, suggesting that these genes are probably involved in the tumorigenesis and progression of NPC.

关 键 词：鼻咽癌 EPSTEIN-BARR病毒 CDNA阵列 人多聚免疫球蛋白受体(hplgR) 基因表达谱 

分 类 号：R739.63[医药卫生—肿瘤]