高产β-胡萝卜素杜氏藻突变株的鉴定与分析  

Mutation and identification of mutant strain of Dunaliella bardawil containing highly in β-carotene

作　　者：孙福征[1] 刘广发[1] 陈启伟[1] 高亚辉[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿癌细胞工程教育部重点实验室,福建厦门361005

出　　处：《大连水产学院学报》2004年第3期230-233,共4页Journal of Dalian Fisheries University

基　　金：福建省科技计划重点项目资助(2003No53)

摘　　要：采用紫外辐射诱导巴氏杜氏藻Dunaliellabardawil突变,经初筛和复筛,初步获得一株巴氏杜氏藻的突变株,对两者从细胞形态特征、β-胡萝卜素含量、蛋白质SDS-PAGE电泳以及总DNA的RAPD扩增等方面进行了比较分析。结果表明,在相同培养条件下,两者均为卵圆形,但原种直径仅为突变后藻株直径的60%,颜色差异明显;突变株的β-胡萝卜素含量为原种的6 2～15 5倍;两者遗传相似系数为0 868,与突变株的一般要求相符。鉴于此,确认其为高产β-胡萝卜素的新突变株,命名为Dunaliellabardawilvariant4-5Sun＆Liu。A β-carotene-riched mutant of Dunaliella bardawil by ultraviolet radiation has been identified. The mutant strain was found to be larger in diameter than D. bardawil was. The content of β-carotene of the mutant strain reached as high as 6.20% of the dry matter and was 6-14 times than the wild-type original one. Furthermore, several new bands of protein (polypeptide) of the mutant was identified by the protein SDS-PAGE, meanwhile some were disappeared. The RAPD test revealed that the genetic similarity between D. bardawil and its mutant strain was 0.868, indicating that this is a mutant from D. bardawil and designated as D. bardawil variant 4-5 Sun & Liu. It may be an excellent strain for the commercial large-scale β-carotene production.

关 键 词：杜氏藻 诱变 Β-胡萝卜素 遗传相似系数 

分 类 号：Q949[生物学—植物学]