三种黄鳝RAPD分析的引物筛选  

The Selection of Polymorphic Primers to Three Species of Monopterus by RAPD Analysis

作　　者：张继平[1] 贺顺连[1] 周孟娇[2] 郭金彪[1] 张绮琼[1] 

机构地区：[1]广东佛山科学技术学院动物科学系,广东佛山528231 [2]湖南农业大学动物科技学院,湖南长沙410128

出　　处：《水产科学》2004年第9期23-25,共3页Fisheries Science

摘　　要：试验用随机扩增多态DNA(RAPD)技术对黄鳝属中产自缅甸的山黄鳝、印度尼西亚的穴黄鳝及中国的黄鳝的混合DNA进行引物筛选。40条引物有35条引物扩增出多态性片段。35条引物共获得334个扩增片段,长度为250～5417bp。The samples of three species of synbranchi form eels,Monopterus cuchia,M.fossorius and M.albus were collected from Yangon of Myanmar,Indonesia and Yuanjiang County, Hunan Province,China respectively.Randomly amplified polymorphic DNA (RAPD) technique was used to analyse the selection of polymorphic primers of the wild grouper population of four individuals from each species.35 out of 40 polymorphic priners amplified diversity.35 primers produced 334 fingerprints with the molecular length varying from 250 bp to 5417 bp.

关 键 词：山黄鳝 穴黄鳝 黄鳝 RAPD 引物筛选 

分 类 号：S917[农业科学—水产科学]