人小细胞肺癌NCI-H446细胞裸鼠异种移植瘤模型的建立  

Xenotransplantation Model of A Human Small Cell Lung Cancer Cell Line——NCI-H446 in Nude Mice

作　　者：林芸秀[1] 吕联煌[1] 林锦娟[1] 陈英玉[1] 

机构地区：[1]福建医科大学附属协和医院血液科福建省血液病研究所,福州350001

出　　处：《实验动物科学与管理》2004年第3期1-3,共3页Laboratory Animal Science & Administration

摘　　要：目的　建立成瘤稳定、潜伏期短的人小细胞肺癌NCI_H4 4 6细胞裸鼠移植瘤模型。方法　初代移植后 ,通过皮下埋块法进行裸鼠体内连续传代移植 ,并对移植瘤的生长、组织形态和超微结构、染色体核型进行观察和检测。结果　通过连续传代移植建立了成瘤稳定、潜伏期短的人小细胞肺癌NCI_H4 4 6细胞裸鼠移植瘤模型。移植瘤的组织形态和超微结构特点符合NCI_H4 4 6细胞特点 。Objective To establish a successful xenotransplantation model of a human small cell lung cancer cell line (NCI_H446) in nude mice. Methods NCI_H446 cells were transplanted subcutaneously into nude mice.After the first generation,the xenografls were transplanted subcutaneously into nude mice with serial passages for three generations.The growth features,histology,ultramicroscope structure and karyology of the successfully transplanted tumors were observed. Results After four generations of transplantation the latent period was shorten, and the spontaneous regression was not observed.The growth curve of the tumor is like “S”.The karyotype of tumor cells belongs to human karyofype.

关 键 词：人 小细胞肺癌 裸鼠 异种移植 移植瘤 病理模型 

分 类 号：R734.2[医药卫生—肿瘤] R-332[医药卫生—临床医学]