水稻淀粉胚乳细胞发育期间程序性死亡的研究  

Studies on the Programmed Cell Death in Rice During Starchy Endosperm Development

作　　者：李睿[1] 兰盛银[2] 徐珍秀[2] 

机构地区：[1]武汉工业学院生物与化学工程系,武汉430023 [2]华中农业大学生命科学院,武汉430070

出　　处：《中国农业科学》2004年第8期1119-1122,i002,i003,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30070363);高等学校博士学科点专项科研基金资助项目(2000050401)

摘　　要：应用透射电子显微镜观察水稻淀粉胚乳细胞核在发育期间的形态变化。结果表明,伴随淀粉胚乳发育进程,其细胞核呈现染色质凝聚这一程序性细胞死亡(PCD)的典型特征。水稻胚乳细胞还出现核变形、核膜破裂、核基质进入胞质乃至核降解形成核残体的现象,细胞核从变形到解体是以一种有序的方式进行,证实水稻淀粉胚乳的细胞死亡是程序性细胞死亡。EVANS BLUE染色结果表明,水稻淀粉胚乳细胞死亡顺序是由胚乳中部向四周扩展。随发育进程,水稻胚乳中的超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性持续下降。对水稻淀粉胚乳中的DNA进行凝胶电泳分析,不呈现典型的DNA梯状条带。结果表明,水稻淀粉胚乳细胞的程序性死亡是一种特殊形式的程序性死亡现象。Morphological variations of the nucleus in starchy endosperm cell were observed by electron-transmisson microscope during endosperm development in rice. Along with the development of the starchy endosperm, the nuclei of the cells showed chromatin condensation, the typical feature of programmed cell death (PCD). The nuclei also showed nucleus deformation, disruption of nuclear envelope, nucleoplasm leaking into the cytoplasm and nucleus disintegration resulting in nuclear residue formation. From the nucleus deformation to the nucleus disintegration, the morphological changes of the nucleus were orderly progressive. This indicated that the cell death of starchy endosperm in rice was programmed cell death. Evans Blue staining observation showed that the cell death was initially detected in the central part of starchy endosperm in rice, then expanded outward. The activities of Superoxide dismutase (SOD) and catalase (CAT) in rice starchy endosperm both descended continuously as development progressed. The analysis of DNA of rice starchy endosperm did not show the presence of DNA laddering. It is concluded that the cell death of starchy endosperm in rice is a special form of PCD.

关 键 词：水稻 淀粉 胚乳细胞 发育期间 程序性死亡 

分 类 号：S511[农业科学—作物学]