芽孢杆菌L-32发酵条件优化及提高原油采收率实验研究  

Laboratory experiment on optimization and application of fermentation condition of bacillus L-32 to enhance oil recovery

作　　者：李清心[1] 康从宝[1] 林建强[1] 王浩[1] 张长铠[1] Li, Qing-Xin; Kang, Cong-Bao; Lin, Jian-Qiang; Wang, Hao; Zhang, Chang-Kai

机构地区：[1]山东大学微生物技术国家重点实验室

出　　处：《石油大学学报（自然科学版）》2004年第3期52-55,140,共4页Journal of the University of Petroleum,China(Edition of Natural Science)

基　　金：教育部 2 0 0 1-2 0 0 3博士点基金资助 ( 2 0 0 0 42 2 12 )

摘　　要：经筛选得到了一株能以原油为碳源进行生长繁殖的菌株 ,初步鉴定为芽孢杆菌 (L 32 ) ,对其发酵条件进行了优化 ,确定出最佳的发酵条件 ,并确定了厌氧条件下以原油为碳源时的培养基成分。通过补加葡萄糖使其浓度保持在8g/L的方法可以提高菌体的浓度。实验结果表明 ,L 2 3可以以原油为碳源生长 ,并能在降低原油粘度的同时使原油培养基内水相的pH值下降。L 2 3能提高实验岩心的原油采收率 ,即在关井 96h后 ,L 2 3可使原油采收率提高 9.6 %左右。A piece of bacillus L-32, which is growing and procreating by taking crude oil as its carbon source, was screened. The fermentation condition to produce L-32 was optimized. The concentration of L-32 could be enhanced by adding glucose with a concentration of 0.8 percent in the medium. The medium for growing L-32 by taking crude oil as carbon source was optimized. The experiment demonstrated that L-32 could grow by taking crude oil as its carbon source and could reduce oil viscosity and produce some acid to lower pH value of culture medium. Under laboratory conditions, L-32 could increase oil recovery in a model reservoir by about 9.6 percent after oil well shut-in for 96 hours.

关 键 词：芽孢杆菌 L-32 提高采收率 原油 微生物采油工艺 发酵液 葡萄糖 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]