益气解毒颗粒干预鼻咽癌细胞HNE1蛋白质表达的研究  

An investigation on tbe proteomics of nasopharyngeal carcinoma cell line HNE1 treated with Qi-boosting toxin-resolving granule

作　　者：唐发清[1] 田道法[2] 龚志军[1] 顾焕华[3] 荆照政[1] 肖志强[1] 唐银[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410078 [2]湖南中医学院第一附属医院 [3]中南大学肿瘤研究所

出　　处：《中国中西医结合耳鼻咽喉科杂志》2004年第3期120-123,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：国家自然科学基金(39770933;30171174;30271678);国家中医药管理局资助项目(95B063;02-03JP29);湖南省教育厅重点资助项目(01A014)

摘　　要：目的 探讨中药复方益气解毒颗粒对鼻咽癌细胞杀伤作用的分子机制。方法 筛选益气解毒颗粒药液对鼻咽癌杀伤的半抑制浓度(IC_(50)),用0.1倍半抑制浓度(0.1×IC_(50))的益气解毒颗粒作用鼻咽癌细胞HNE1 96h,用固相pH梯度双向凝胶电泳分离益气解毒颗粒处理和未处理的鼻咽癌细胞HNE1的总蛋白质,银染显色,PDQuest 2-de软件分析差异蛋白质点。结果 益气解毒颗粒药液杀伤鼻咽癌细胞HNE1的半抑制浓度为6.25mg/ml;双向电泳图像分析益气解毒颗粒药物处理组图像的平均蛋白质数为1120±89,未处理组的平均蛋白质数为1281±102;差异蛋白质有673个,其中有218个表达增强,有455个蛋白质表达下降。结论 益气解毒颗粒对鼻咽癌细胞的杀伤作用这些差异表达蛋白质有关,这些差异蛋白质有待质谱分析鉴定,为中药作用的分子机理研究提供了新的思路。Objective To investigate the molecular mochanism of Qi-Boosting Toxin Resolving Granule (QBTRG)in the inhibition of nasopharyngeal carcinoma cell line HNE1 based on the techniques of proteomics. Methods The IC_(50) of QBTRG killing HNE1 cells was determined by MTT at first. Then, HNE1 cells were treated with QBTRG at the concentration of 0.1×IC_(50) for 96 hours. After this step, the cells were collected and the total protein was extracted from these cells. By two-dimension clec- trophoresis, the proteins were separated and protein profiles were obtained. Followed was an analysis on the differently expressed proteins by PDQuest2-de microsolft on the basis of protein profile comparison between two groups of HNE1 cells treated with QB- TRG and those not treated at all. Results The IC_(50) of QBTRG on NHE1 cells was 6.25mg/ml. The mean number of protein spots was 1120±89 for treated HNE1 cells with QBTRG and that was 1281±102 for the untreated HNE1 cells. Therefore, the dif- ferently expressed protein spots were 673, among them 218 being up-regulated and 455 being down-regulated in their expression in treated HNE1 cells. Conclusions The in- hibitory effects of QBTRG on nasopharyngeal careinoma cells may be closely associated with the pattern of these differently expressed proteins. The physical and chemical prop- erties of these proteins can be identified by the means of MS. Then, the data got from this identification should be helpful for the further investigation on the molecular mecha- nism of QBTRG in the inhibition of this kind of cancer cells.

关 键 词：益气解毒颗粒 鼻咽癌 细胞HNE1蛋白质 表达 双向电泳 

分 类 号：R739.63[医药卫生—肿瘤]