大鼠角膜碱烧伤不同时期MMP-1及其抑制剂的表达  

Expression of MMP-1 and its inhibitor in alkali-burned rat cornea

作　　者：王依鹭[1] 洪晶[2] 

机构地区：[1]深圳市罗湖区人民医院眼科,深圳518001 [2]中国医科大学第一附属医院眼科

出　　处：《中国实用眼科杂志》2004年第8期660-663,共4页Chinese Journal of Practical Ophthalmology

摘　　要：目的　探讨碱烧伤后不同时期基质金属蛋白酶 -1(MMP -1)及其组织型抑制剂(TIMP -1)的表达及作用。方法　建立大鼠角膜碱烧伤模型 ,应用免疫组化方法检测MMP -1及TIMP -1在碱烧伤后不同时期角膜内的表达。结果　免疫组化显示 :正常大鼠角膜组织中无MMP -1及其抑制剂的表达。实验组MMP -1于伤后第 1天开始升高 ,14天达到最高 ,此后呈现下降趋势 ,但仍高于正常对照组。而TIMP -1早期有所表达 ,而后活性逐渐下降 ,伤后第 14天降至最低水平 ,此后又再度上升 ,2 1天时达到最高。结论　角膜溶解高峰期出现于碱烧伤后第 14天。此时MMP -1分泌达高峰 ,TIMP -1分泌达到最低点。角膜溶解的临床病理过程与MMP -1及TIMP -1的分泌密切相关。Objective To investigate the expression and of th MMP-1 in the cornea and its inhibitor in the different period of the development of alkali burn and functions.Methods The rat corneal alkali burn model was establishedand theexpression of MMP 1 and TIMP 1 was studied by immunohistochemistry.Results By immunohistochemistry,no MMP-1 and its inhibitor′s expression were found in the normal rat cornea.Increased expression of MMP-1 was found on the 1 st day,being higghest on the 14th day,then decreased slowly,however it was still higher than the normal.TIMP-1 expressed in the early phrase.But later,its activity droped step by step.TIMP-1 reached the lowest level on the 14th day.After that,it began to increase and being the highest levels seen on the 21th day.Conclusions The high peak of corneal dissolving was found on the 14th day after alkaline burn,on the same time,the excrete of MMP-1 reached the high peak,the excrete of TIMP-1 reached the lowest level.The clinical process of the corneal dissoliving has close connection with the excrete of MMP-1 and TIMP-1.

关 键 词：大鼠 角膜碱烧伤 MMP-1 抑制剂 表达 

分 类 号：R779.1[医药卫生—眼科]