化学物质的Ah受体效应重组基因酵母检测法的优化  

Modification and Application of Recombinant Yeast Bioassay for Measuring the AhR Ligand Activity

作　　者：王伟[1] 胡建英[1] 顾炜旻[1] 刘燕花[1] 

机构地区：[1]北京大学环境学院,北京100871

出　　处：《环境科学》2004年第4期155-158,共4页Environmental Science

基　　金：国家杰出青年科学基金项目 ( 4 992 5 10 3 ) ;国家 863计划项目 ( 2 0 0 2AA60 114 0 6)

摘　　要：接有芳香烃受体 (AhR)基因和芳香烃受体核转位子蛋白 (ARNT)基因的酵母Saccharomycescerevisiae菌株是测定化学物质的Ah受体效应的方法之一 .本实验通过优化各个实验条件 ,发现用 0 2 %葡萄糖作前培养液培养菌 2 4h ,用 2 %半乳糖作化学物质暴露时的培养液 ,暴露在玻璃管时 ,暴露时间从原来的 1 8h缩短到 8h .并用已经优化的方法测定了六氯苯和五氯苯的Ah受体效应 ,得出六氯苯和五氯苯相对于TCDD的毒性等当量值 (TEF)分别为 0 0 1 86 2 9和 0 0 0 0 2 94 .A recombinant yeast bioassay,a yeast Saccharomyces cerevisiae, in which the human AhR and ARNT complex are coexpressed,is one of the methods to screen the active AhR agonist.In this study,the original agonist test was modified.The exposure time was reduced from 18 to 8 hours when experiment was under the following conditions:①the yeast was cultured in 0 2% glucose medium for 24 hours;②chemical exposure was carried in 2% galactose medium in glass tube.Finally,the AhR acitivity of hexachlorobenzene and pentachlorobenzene were assessed,and their toxicity equivalent factors were found to be 0 018629 and 0 000294,respectively.

关 键 词：重组基因酵母检测法 Ah受体效应 六氯苯 五氯苯 

分 类 号：X17[环境科学与工程—环境科学]