假单胞菌DN-1再生NO络合吸收液的特性  

Properties of Pesudomonas sp. DN-1 in Reduction of Nitric Oxide Chelate Absorption Solution

作　　者：荆国华[1] 李伟[1] 施耀[1] 马碧瑶[1] 谭天恩[1] 

机构地区：[1]浙江大学环境工程研究所,杭州310027

出　　处：《环境科学》2004年第4期163-166,共4页Environmental Science

基　　金：国家自然科学基金资助项目 ( 2 0 1760 5 2 ) ;教育部留学回国人员科研启动基金资助项目

摘　　要：FeⅡ(EDTA)络合吸收脱除NO吸收速率快 ,吸收容量大 ,但是存在吸收液再生方面的缺陷 .实验用自行分离得到的假单胞菌DN 1 ,将吸收液中的FeⅡ(EDTA)NO还原为N2 而进行再生 .考察了不同碳源种类、碳源量、菌种量、pH值、温度、络合物浓度等条件下该菌种对FeⅡ(EDTA)NO的还原情况 .结果表明 ,葡萄糖比醋酸钠、柠檬酸三钠和乙醇等更适合于作为该体系的外加碳源 .当FeⅡ(EDTA)NO浓度为 6 5 0mmol·L-1 时 ,添加 2 5 0mg·L-1 的葡萄糖就能满足微生物的反应需要 ;DN 1还原适宜温度范围为 4 0～ 4 5℃ ,适宜pH值为 6 9～ 7 2 ;还原速率随菌体接种量的增多而加快 ;而FeⅡ(EDTA)NO浓度小于 1 1 8mmol·L-1 时 ,还原速率随着络合物浓度的增加而增加 ;超过这一浓度 ,还原速率不再随络合物浓度的增加而增加 。Metal chelate absorption is deemed as a promising method of NO removal in FGD system,but the difficulty in the regeneration of the absorption solution hinders its further development.An original method with microbial reduction is proposed in this paper.With the adding of Psudomonas sp.DN-1,which was newly isolated from wastewater treatment plant,Fe Ⅱ (EDTA)NO will be reduced to the environmentally benign gaseous product of N 2,and thus Fe Ⅱ (EDTA) was regenerated simultaneously.The effects of the types and amount of carbon source,Fe Ⅱ (EDTA)NO concentration,pH,temperature and the biomass inoculation on bio-reduction efficiency were investigated.The results showed that the microorganism exhibited good performance on bio-reduction of Fe Ⅱ (EDTA)NO with the carbon sources of glucose.250 mg·L -1 glucose was enough for microorganism to reduce 6 50 mmol·L -1 Fe Ⅱ (EDTA)NO completely.The rate of Fe Ⅱ (EDTA)NO reduction did not increase with adding a larger amount of carbon source.The bio-reduction could be achieved efficiently among the temperature range of 40～45℃ and a pH range of 6 9～7 2.The bio-reduction rate increased with the increasing of biomass inoculation.When Fe Ⅱ (EDTA)NO concentration is less than 11 8 mmol·L -1 ,the reduction rate increased as the concentration increases,while the concentration is over 11 8 mmol·L -1 ,the reduction rate keeps constant.

关 键 词：NO吸收液 微生物还原 NOx 假单胞菌 

分 类 号：X701[环境科学与工程—环境工程]