荧光定量PCR技术检测浆膜腔积液中CEA-mRNA对恶性肿瘤的诊断价值  

Diagnostic significance of CEA-mRNA detection by RT-PCR for carcinoma

作　　者：吕蕾[1] 蔡菊芬[1] 冯建国[2] 顾怡生[3] 

机构地区：[1]浙江省肿瘤医院化疗科,浙江杭州310022 [2]浙江省肿瘤研究所,浙江杭州310022 [3]杭州师范学院医学院生化教研室,浙江杭州310012

出　　处：《肿瘤防治杂志》2004年第8期859-861,共3页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :通过定量检测浆膜腔积液中CEA mRNA的拷贝数 ,探讨其对恶性积液的诊断价值。方法 :采用荧光定量RT PCR技术分别检测经临床证实的 3 1例恶性浆膜腔积液和 2 8例良性浆膜腔积液样本中CEA mRNA拷贝数 ;所有病例均行脱落细胞涂片检测。结果 :3 1例恶性积液样本中CEA mRNA阳性率为 74 2 % ( 2 3 /3 1) ,CEA mRNA拷贝数在 1 2 2 693 7 5 /mL中位数为 76拷贝 /mL ,脱落细胞检测阳性率为 3 8 7% ( 12 /3 1) ;良性浆膜腔积液样本中CEA mRNA阳性率为 14 3 % ( 4 /2 8) ,拷贝数在 1 1～ 5 7/mL ,中位数为 2 3拷贝 /mL。结论 :荧光定量PCR技术检测积液中CEA mRNA的拷贝数对恶性积液有较高的敏感性 。OBJECTIVE:To explore the diagnostic significance of quantitative detection of CEA-mRNA for malignant dropsy of serous cavity. METHODS: CEA-mRNA were detected with fluorescence quantitative polymerase chain reaction in 31 malignant and 28 nonmalignant dropsy samples respectively.All samples were verified by exfoliative cells.RESULTS: In malignant and nonmalignant dropsy samples,positive rate of CEA-mRNA were 74.2% (23/31)and 14.3% (4/28),the value of CEA-mRNA were 1.2-26937.5 copy/mL and 1.1-5.7 copy/mL,the median were 76 copy/mL and 2.3 copy/mL respectively,while positive rate of exfoliative cells was 38.7% (12/31) in 31 malignant dropsy samples.CONCLUSIONS: CEA-mRNA detection with fluorescence quantitative polymerase chain reaction in dropsy presents high sensitivity and is prospective in clinical practice.

关 键 词：荧光定量PCR 癌胚抗原信使核糖核酸 浆膜腔积液 

分 类 号：R730.4[医药卫生—肿瘤]