周期性牵张体外培养面颌成肌细胞肌浆网Ca^(2+)-Mg^(2+)ATP酶活性及其mRNA变化的研究  

Changes of Sarco(endo)plasmic Reticulum Ca^(2+)-Mg^(2+)-ATPase Activity of Cultured Myoblast Induced by Cyclic Stretch

作　　者：车晓霞[1] 曾宏 罗颂椒[3] 

机构地区：[1]首都医科大学北京口腔医院正畸科,北京100050 [2]成都地奥集团,四川成都610000 [3]四川大学华西口腔医院正畸科,四川成都610041

出　　处：《华西口腔医学杂志》2004年第4期281-283,286,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (30 1 71 0 2 4 )

摘　　要：目的　观察牵张引起的成肌细胞内Ca2 + _Mg2 + ATP酶活性及mRNA变化 ,探求口腔正畸功能矫形治疗中肌肉改建机理 ,为正畸临床治疗及防止复发提供理论性指导。方法　采用四点弯曲加力装置 ,使体外培养的SD大鼠面颌部成肌细胞发生牵张变形后 ,无机磷 -钼酸铵直接比色法测定加力后不同时段Ca2 + _Mg2 + ATP酶活性 ,RT_PCR测定肌浆网Ca2 + _Mg2 + ATP酶mRNA变化。结果　SD大鼠体外培养成肌细胞在加力变形后的 4h内Ca2 + _Mg2 + ATP酶活性显著降低 ,8h到 2 4h期间明显升高 ,4 8h基本接近对照组水平 ;此外 ,RT_PCR结果显示成肌细胞受牵张力后Ca2 + _Mg2 + ATP酶mRNA水平在各时间段组均较对照组升高 ,其中以 2h和 4 8h段组升高最明显。结论　随着受力时间的延长 ,成肌细胞发生了适应性变化 ,Ca2 + _Mg2 + ATP酶活性变化的控制发生在基因翻译前水平 ,Ca2 + _Mg2 + ATP酶活性及其mRNA变化会直接影响对细胞内游离Ca2 + 浓度的调节 ,继而引起细胞内发生的一系列生物学反应的变化。Objective To evaluate the effect of stretch on sarco(endo)plasmic reticulum Ca+{2+}-Mg+{2+} ATPases activity and mRNA level and study the remodeling reaction of muscle in a variety of mechanical environments.Methods Myoblast from maxillofacial skeletal muscle of one-week-old male Sprague-Dawley rat was cultured and stretched cyclicly using a four-point bend device. Inorganic Phosphorus test was used to compare the activity of Ca+{2+} -Mg+{2+}ATPases of myoblast before and after stretch. RT-PCR was also used to observe the Ca+{2+}-Mg+{2+}ATPases mRNA level.Results The activity of Ca+{2+}-Mg+{2+}ATPases of myoblast down regulated significantly in 4 hours. During the period of 8 hours to 24 hours, up-regulation followed then returned to control level at the 48 hour point. RT-PCR showed that Ca+{2+}-Mg+{2+} ATPases mRNA level were elevated by stretch, particularly at 2 hour and 48 hour point.Conclusion The results suggested a transcriptional control of Ca2+-Mg2+ ATPases activity was involved in the muscle remodeling process induced by stretch.

关 键 词：牵张 成肌细胞 Ca^2+-Mg^2+ATP酶 

分 类 号：R783.5[医药卫生—口腔医学]