咬合创伤致兔咬肌线粒体钙离子和二、三磷酸腺苷含量的改变  

Changes of Mitochondrial Calcium, Adenosine Triphosphate and Adenosine Diphosphate Contents Induced by Occlusal Trauma in Rabbit′s Masseter Muscles

作　　者：孙淑贞[1] 汲平[1] 来庆国[2] 王惠[3] 祁冬[3] 王喜军[3] 王梁[3] 王明臻[3] 

机构地区：[1]山东大学口腔医院修复科 [2]山东大学第二医院口腔科,山东济南250012 [3]山东大学口腔医院

出　　处：《华西口腔医学杂志》2004年第4期313-316,共4页West China Journal of Stomatology

摘　　要：目的　研究线粒体Ca2 + 超载及能量代谢在咬合创伤所致咀嚼肌功能紊乱中所起的作用。方法　用原子发射光谱法检测兔咬肌线粒体Ca2 + 含量 ;用高效液相色谱法检测线粒体二磷酸腺苷 (ADP)、三磷酸腺苷 (ATP)含量。结果　① 10d组、2 0d组的实验组板侧和 2 0d组的板对侧的咬肌线粒体Ca2 + 含量明显升高 (P <0 0 5 ) ;②2 0d组的实验组板侧的咬肌线粒体ATP含量低于对照组和板对侧 (P <0 0 5 )。③实验组板侧的咬肌线粒体Ca2 + 与ATP含量之间呈负相关 (r=_0 780 ,P <0 0 5 )。结论　线粒体Ca2 + 超载通过抑制ATP合成使咀嚼肌细胞出现能量代谢紊乱 ,可能是咬合创伤致咀嚼肌功能紊乱的主要作用机制。Objective To study the role of Ca+{2+} overload and energy metabolism in mitochondria in masticatory muscle dysfunctional induced by occlusal trauma.Methods Mitochondrial Ca+{2+} contents were measured with atomic emission spectrophotometer. Mitochondrial ATP and ADP contents were measured with high performance liquid chromatography.Results ① Mitochondrial Ca+{2+} contents of masseter muscle ipsilateral to mental splint in ten and twenty days′ experimental groups and that contralateral to mental splint in twenty days′ experimental group increased significantly (P<0.05). ②Mitochondrial ATP contents of masseter muscle ipsilateral to mental splint in experimental groups were higher than that in control groups and contralateral to mental splint after twenty days. ③Mitochondrial Ca+{2+} contents of masseter muscle ipsilateral to mental splint were significantly negatively correlated to the mitochondrial ATP contents(r=-0.780, P<0.05).Conclusion Ca+{2+} overload in mitochondria depresses ATP production, which results in energy metabolism disorder in masticatory muscle cells. It may play an important role in the mechanism that occlusal trauma results in masticatory muscle dysfunction.

关 键 词：咬合创伤 咀嚼肌功能紊乱 钙离子 二磷酸腺苷 三磷酸腺苷 

分 类 号：R782.6[医药卫生—口腔医学]