压力对髁突软骨细胞一氧化氮合酶活力和前列腺素分泌的影响及其G蛋白在其中作用的研究  

Effects of pressure on the nitric oxide synthase activity and PGF_1α level in rabbit mandrabular condylar chondrocytes and function of the G protein in it

作　　者：张旻 王美青[1] 王景杰[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2004年第8期433-438,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目 ( 30 0 0 0 0 35 )

摘　　要：目的 :探讨髁突软骨细胞一氧化氮合酶 (NOS)活力及前列腺素 (PG)分泌随机械力的变化过程及其G蛋白在其中的作用。方法 :体外培养髁突软骨细胞 ,分为无加压无G蛋白拮抗剂、90kPa加压无G蛋白拮抗剂、6 0kPa加压无G蛋白拮抗剂、无加压 10 0 μmol/LG蛋白拮抗剂、90kPa加压 10 0 μmol/LG蛋白拮抗剂、6 0kPa加压 10 0 μmol/LG蛋白拮抗剂 6组。在处理后 1、4、8、12h ,取上清液 ,采用化学比色法及放免分析法进行NOS酶活力及PGF1α含量检测。结果 :90kPa静压力可刺激髁突软骨NOS活力的升高和PGF1α合成的下降 ,G蛋白拮抗剂可逆转该作用 ,且逆转效应时相与拮抗剂浓度有关。高浓度拮抗剂在 1h时对NOS及PG的逆转效果最明显 ,而低浓度拮抗剂在 8h才出现对PGF1α分泌的逆转效应 ,但对NOS活力在观察期内无显著影响。6 0kPa静压力会引起NOS活性的下降和PGF1α合成的增加 ,G蛋白拮抗剂可部分削弱该作用 ,其削弱效应时相仍与拮抗剂浓度有关。高浓度拮抗剂在 1h即可出现明显的削弱作用 ,低浓度拮抗剂在 4h后出现对 6 0kPa压力下NOS降低的削弱作用 ,而 8h后才出现对 6 0kPa压力促进PGF1α释放的削弱效应。结论 :90kPa静压力作用下可刺激髁突软骨NOS活力的升高和PGF1α合成的下降 ,而 6 0kPa静压力反而会引起NOS活性的下?AIM:To investigate the change of the nitric oxide synthase activity and PGF()_1α level under the mechanical pressure in rabbit mandibular condylar chondrocytes (MCC) and the role of the G protein in it.METHODS:In vitro cultured MCC from six two-week-old New Zealand rabbits were incubated and divided into six groups with different pressure enviroments and different concentrations of G protein inhibitor.At 1,4,8 and 12 h after the corresponding treatment of each group,the culture supernatant were collected.The spectrophotometric method was applied to determine the activity of nitric oxide synthase (NOS).Radioimmunology assay (RIA) was adopted for PGF_1α determination.RESULTS:Static mechanical pressure of 90kPa could cause increase of NOS activity and decrease of PGF_1α composition. G protein inhibitor could counteract the effect, which was related with the concentration of inhibitor. The reversing effect was most distinct at 1 h for both NOS and PG with higher inhibitor concentration and 8 h for PGF_1α with lower inhibitor concentration, under which there was no significant effect for NOS activity. Whereas, static pressure of 60kPa could cause decrease of NOS activity and increase of PGF_1α composition. G protein inhibitor could weaken the effect, which was also related with concentration of the inhibitor. The weakening effect was most distinct at 1 h with higher inhibitor concentration for both NOS and PGF_1α. With lower inhibitor concentration, the weakening effect was most distinct at 4 h for NOS activity and 8 h for PGF_1α synthesis.CONCLUSION:Static pressure of 90kPa could cause increase of NOS activity and decrease of PGF_1α composition while the effects of 60 kPa pressure was in opposition to that. G protein participates in both of the different regulation process.

关 键 词：髁突软骨细胞 压力 前列腺素 一氧化氮合酶 G蛋白 

分 类 号：R780.2[医药卫生—口腔医学]